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Ammonium-Dependent Shortening of CLS in Yeast Cells Starved for Essential Amino Acids Is Determined by the Specific Amino Acid Deprived, through Different Signaling Pathways
Joint Authors
Santos, Júlia
Sousa, Maria João
Leão, Cecília
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-08-26
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Ammonium (NH4+) leads to chronological life span (CLS) shortening in Saccharomyces cerevisiae BY4742 cells, particularly evident in cells starved for auxotrophy-complementing amino acids (leucine, lysine, and histidine) simultaneously.
Here, we report that the effect of NH4+ on aging yeast depends on the specific amino acid they are deprived of.
Compared with no amino acid starvation, starvation for leucine alone or in combination with histidine resulted in the most pronounced NH4+-induced CLS shortening, whereas starvation for lysine, alone or in combination with histidine resulted in the least sensitivity to NH4+.
We also show that NH4+-induced CLS shortening is mainly mediated by Tor1p in cells starved for leucine or histidine but by Ras2p in cells starved for lysine, and in nonstarved cells.
Sch9p protected cells from the effect of NH4+ under all conditions tested (starved or nonstarved cells), which was associated with Sch9p-dependent Hog1p phosphorylation.
Our data show that NH4+ toxicity can be modulated through manipulation of the specific essential amino acid supplied to cells and of the conserved Ras2p, Tor1p, and Sch9p regulators, thus providing new clues to the development of environmental interventions for CLS extension and to the identification of new therapeutic targets for diseases associated with hyperammonemia.
American Psychological Association (APA)
Santos, Júlia& Leão, Cecília& Sousa, Maria João. 2013. Ammonium-Dependent Shortening of CLS in Yeast Cells Starved for Essential Amino Acids Is Determined by the Specific Amino Acid Deprived, through Different Signaling Pathways. Oxidative Medicine and Cellular Longevity،Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-450781
Modern Language Association (MLA)
Santos, Júlia…[et al.]. Ammonium-Dependent Shortening of CLS in Yeast Cells Starved for Essential Amino Acids Is Determined by the Specific Amino Acid Deprived, through Different Signaling Pathways. Oxidative Medicine and Cellular Longevity No. 2013 (2013), pp.1-10.
https://search.emarefa.net/detail/BIM-450781
American Medical Association (AMA)
Santos, Júlia& Leão, Cecília& Sousa, Maria João. Ammonium-Dependent Shortening of CLS in Yeast Cells Starved for Essential Amino Acids Is Determined by the Specific Amino Acid Deprived, through Different Signaling Pathways. Oxidative Medicine and Cellular Longevity. 2013. Vol. 2013, no. 2013, pp.1-10.
https://search.emarefa.net/detail/BIM-450781
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-450781