Individual Osmotic Fragility Distribution : A New Parameter for Determination of the Osmotic Properties of Human Red Blood Cells
Joint Authors
Chludzińska, Ludmiła
Walski, Tomasz
Komorowska, Małgorzata
Witkiewicz, Wojciech
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-01-05
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
The aim of our experiments was to characterise and to validate the osmotic fragility test when applied to human blood samples with no significant alterations of osmotic fragility but with a differentiating shape of the haemolysis curve.
All experiments were carried out on human erythrocytes taken from the Regional Centre of Blood Donation and Blood Therapy in Wrocław.
The washed erythrocytes were exposed to near-infrared radiation (NIR) or ozonated, and the osmotic fragility test was applied.
The osmotic fragility, calculated from the experimental haemolysis curve for the control and cells irradiated for 15 min, is the same within the empirical error.
Calculation of the first derivative of the haemolysis curve allowed us to visualise the changes in osmotic fragility distribution after exposure to NIR.
By contrast, significant changes both to the osmotic fragility value and the distribution of osmotic properties were observed after an erythrocytes ozonation procedure.
Description of cell osmotic properties requires at least two parameters—the value of osmotic fragility and the slope of the haemolysis curve in the region where absorbance sharply increases due to cell haemolysis.
American Psychological Association (APA)
Walski, Tomasz& Chludzińska, Ludmiła& Komorowska, Małgorzata& Witkiewicz, Wojciech. 2014. Individual Osmotic Fragility Distribution : A New Parameter for Determination of the Osmotic Properties of Human Red Blood Cells. BioMed Research International،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-450791
Modern Language Association (MLA)
Walski, Tomasz…[et al.]. Individual Osmotic Fragility Distribution : A New Parameter for Determination of the Osmotic Properties of Human Red Blood Cells. BioMed Research International No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-450791
American Medical Association (AMA)
Walski, Tomasz& Chludzińska, Ludmiła& Komorowska, Małgorzata& Witkiewicz, Wojciech. Individual Osmotic Fragility Distribution : A New Parameter for Determination of the Osmotic Properties of Human Red Blood Cells. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-450791
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-450791