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A Validated LC Method for Separation and Determination of Tetralone-4-O-β-D-Glucopyranoside and 4-Hydroxy-α-Tetralone in Ammannia multiflora
Joint Authors
Verma, Ram K.
Srivastava, Santosh K.
Upadhyay, Harish C.
Source
Chromatography Research International
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-4, 4 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-20
Country of Publication
Egypt
No. of Pages
4
Main Subjects
Abstract EN
A rapid, sensitive, and reproducible RP-HPLC method was developed for the determination of two constituents in Ammannia multiflora, namely, tetralone-4-O-β-D-glucopyranoside (1) and 4-hydroxy-α-tetralone (2).
The samples were separated on a Spherisorb ODS2 column (250×4.6 mm, i.d., 10 μm) and binary elution of water and methanol (2 : 3) with flow rate of 0.8 mL/min at λ 254 nm.
The LOD and LOQ were found 0.05 and 0.18 μg/mL for compound 1 and 0.06 and 0.18 μg/mL for compound 2, respectively.
All calibration curves showed good linearity (r2>0.999) within test ranges for both the analytes.
The RSD values for intra-and inter-day precisions were less than 1.1%.
The successful application of the developed method on five different samples revealed an average 0.0206% and 0.7636% (w/w) of compounds 1 and 2, respectively in A.
multiflora indicating that the developed LC assay method may be readily utilized as a quality control method for the plant.
American Psychological Association (APA)
Upadhyay, Harish C.& Verma, Ram K.& Srivastava, Santosh K.. 2012. A Validated LC Method for Separation and Determination of Tetralone-4-O-β-D-Glucopyranoside and 4-Hydroxy-α-Tetralone in Ammannia multiflora. Chromatography Research International،Vol. 2012, no. 2012, pp.1-4.
https://search.emarefa.net/detail/BIM-450798
Modern Language Association (MLA)
Upadhyay, Harish C.…[et al.]. A Validated LC Method for Separation and Determination of Tetralone-4-O-β-D-Glucopyranoside and 4-Hydroxy-α-Tetralone in Ammannia multiflora. Chromatography Research International No. 2012 (2012), pp.1-4.
https://search.emarefa.net/detail/BIM-450798
American Medical Association (AMA)
Upadhyay, Harish C.& Verma, Ram K.& Srivastava, Santosh K.. A Validated LC Method for Separation and Determination of Tetralone-4-O-β-D-Glucopyranoside and 4-Hydroxy-α-Tetralone in Ammannia multiflora. Chromatography Research International. 2012. Vol. 2012, no. 2012, pp.1-4.
https://search.emarefa.net/detail/BIM-450798
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-450798