Fertilization Rate and Number of Embryos on Day 2 after Intrauterine and Deep Intrauterine Insemination Using Frozen-Thawed Boar Semen in Multiparous Sows

Abstract EN

The present study determines fertilization rate and number of embryos on Day 2 after intrauterine insemination (IUI) and deep intrauterine insemination (DIUI) using frozen-thawed (FT) boar semen in multiparous sows.

Twelve crossbred Landrace × Yorkshire multiparous sows were included.

The sows were inseminated at 24 h after oestrus detection and reinseminated every 12 h until ovulation took place.

The inseminations were conducted using IUI with 2×109 FT sperm per dose (n=6) and DIUI with 1×109 FT sperm per dose (n=6).

The sows were slaughtered at 45.1±7.2 h after ovulation.

Embryos and unfertilized oocytes were flushed from the oviducts.

IUI yielded a better fertilization rate than DIUI (66.0% versus 31.0%, P<.001).

The number of embryos was 13.5±2.7 and 6.6±3.2 embryos/sow in IUI and DIUI groups, respectively (P=.08).

The proportion of sows having unilateral fertilization was higher in the DIUI (3/5) than the IUI group (1/6).

In conclusion, IUI with at least 2×109 total number of FT boar spermatozoa is recommended.