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Effect of Alcohol Structure on the Optimum Condition for Novozym 435-Catalyzed Synthesis of Adipate Esters
Joint Authors
Basri, Mahiran
Abdul Rahman, Mohd Basyaruddin
Chaibakhsh, Naz
Source
Biotechnology Research International
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-12-27
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Information Technology and Computer Science
Abstract EN
Immobilized Candida antarctica lipase B, Novozym 435, was used as the biocatalyst in the esterification of adipic acid with four different isomers of butanol (n-butanol, sec-butanol, iso-butanol, and tert-butanol).
Optimum conditions for the synthesis of adipate esters were obtained using response surface methodology approach with a four-factor-five-level central composite design concerning important reaction parameters which include time, temperature, substrate molar ratio, and amount of enzyme.
Reactions under optimized conditions has yielded a high percentage of esterification (>96%) for n-butanol, iso-butanol, and sec-butanol, indicating that extent of esterification is independent of the alcohol structure for primary and secondary alcohols at the optimum conditions.
Minimum reaction time (135 min) for achieving maximum ester yield was obtained for iso-butanol.
The required time for attaining maximum yield and also the initial rates in the synthesis of di-n-butyl and di-sec-butyl adipate were nearly the same.
Immobilized Candida antarctica lipase B was also capable of esterifying tert-butanol with a maximum yield of 39.1%.
The enzyme is highly efficient biocatalyst for the synthesis of adipate esters by offering a simple production process and a high esterification yield.
American Psychological Association (APA)
Abdul Rahman, Mohd Basyaruddin& Chaibakhsh, Naz& Basri, Mahiran. 2011. Effect of Alcohol Structure on the Optimum Condition for Novozym 435-Catalyzed Synthesis of Adipate Esters. Biotechnology Research International،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-450883
Modern Language Association (MLA)
Abdul Rahman, Mohd Basyaruddin…[et al.]. Effect of Alcohol Structure on the Optimum Condition for Novozym 435-Catalyzed Synthesis of Adipate Esters. Biotechnology Research International No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-450883
American Medical Association (AMA)
Abdul Rahman, Mohd Basyaruddin& Chaibakhsh, Naz& Basri, Mahiran. Effect of Alcohol Structure on the Optimum Condition for Novozym 435-Catalyzed Synthesis of Adipate Esters. Biotechnology Research International. 2011. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-450883
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-450883