Isorhynchophylline Protects PC12 Cells Against Beta-Amyloid-Induced Apoptosis via PI3KAkt Signaling Pathway
Joint Authors
Mao, Qing-Qiu
Ip, Paul Siu-Po
Chen, Jian-Nan
Lai, Xiao-Ping
Lin, Zhi-Xiu
Xian, Yan-Fang
Su, Zi-Ren
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-06
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The neurotoxicity of amyloid-β (Aβ) has been implicated as a critical cause of Alzheimer’s disease.
Isorhynchophylline (IRN), an oxindole alkaloid isolated from Uncaria rhynchophylla, exerts neuroprotective effect against Aβ25–35-induced neurotoxicity in vitro.
However, the exact mechanism for its neuroprotective effect is not well understood.
The present study aimed to investigate the molecular mechanisms underlying the protective action of IRN against Aβ25–35-induced neurotoxicity in cultured rat pheochromocytoma (PC12) cells.
Pretreatment with IRN significantly increased the cell viability, inhibited the release of lactate dehydrogenase and the extent of DNA fragmentation in Aβ25–35-treated cells.
IRN treatment was able to enhance the protein levels of phosphorylated Akt (p-Akt) and glycogen synthase kinase-3β (p-GSK-3β).
Lithium chloride blocked Aβ25–35-induced cellular apoptosis in a similar manner as IRN, suggesting that GSK-3β inhibition was involved in neuroprotective action of IRN.
Pretreatment with LY294002 completely abolished the protective effects of IRN.
Furthermore, IRN reversed Aβ25–35-induced attenuation in the level of phosphorylated cyclic AMP response element binding protein (p-CREB) and the effect of IRN could be blocked by the PI3K inhibitor.
These experimental findings unambiguously suggested that the protective effect of IRN against Aβ25–35-induced apoptosis in PC12 cells was associated with the enhancement of p-CREB expression via PI3K/Akt/GSK-3β signaling pathway.
American Psychological Association (APA)
Xian, Yan-Fang& Lin, Zhi-Xiu& Mao, Qing-Qiu& Chen, Jian-Nan& Su, Zi-Ren& Lai, Xiao-Ping…[et al.]. 2013. Isorhynchophylline Protects PC12 Cells Against Beta-Amyloid-Induced Apoptosis via PI3KAkt Signaling Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-450890
Modern Language Association (MLA)
Xian, Yan-Fang…[et al.]. Isorhynchophylline Protects PC12 Cells Against Beta-Amyloid-Induced Apoptosis via PI3KAkt Signaling Pathway. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-450890
American Medical Association (AMA)
Xian, Yan-Fang& Lin, Zhi-Xiu& Mao, Qing-Qiu& Chen, Jian-Nan& Su, Zi-Ren& Lai, Xiao-Ping…[et al.]. Isorhynchophylline Protects PC12 Cells Against Beta-Amyloid-Induced Apoptosis via PI3KAkt Signaling Pathway. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-450890
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-450890