AP-1 as a Regulator of MMP-13 in the Stromal Cell of Giant Cell Tumor of Bone
Joint Authors
Mak, Isabella W. Y.
Popovic, Snezana
Turcotte, Robert E.
Singh, Gurmit
Ghert, Michelle
Source
Biochemistry Research International
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-02-27
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Matrix-metalloproteinase-13 (MMP-13) has been shown to be an important protease in inflammatory and neoplastic conditions of the skeletal system.
In particular, the stromal cells of giant cell tumor of bone (GCT) express very high levels of MMP-13 in response to the cytokine-rich environment of the tumor.
We have previously shown that MMP-13 expression in these cells is regulated, at least in part, by the RUNX2 transcription factor.
In the current study, we identify the expression of the c-Fos and c-Jun elements of the AP-1 transcription factor in these cells by protein screening assays and real-time PCR.
We then used siRNA gene knockdown to determine that these elements, in particular c-Jun, are upstream regulators of MMP-13 expression and activity in GCT stromal cells.
We conclude that there was no synergy found between RUNX2 and AP-1 in the regulation of the MMP13 expression and that these transcription factors may be independently regulated in these cells.
American Psychological Association (APA)
Mak, Isabella W. Y.& Turcotte, Robert E.& Popovic, Snezana& Singh, Gurmit& Ghert, Michelle. 2011. AP-1 as a Regulator of MMP-13 in the Stromal Cell of Giant Cell Tumor of Bone. Biochemistry Research International،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-450991
Modern Language Association (MLA)
Mak, Isabella W. Y.…[et al.]. AP-1 as a Regulator of MMP-13 in the Stromal Cell of Giant Cell Tumor of Bone. Biochemistry Research International No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-450991
American Medical Association (AMA)
Mak, Isabella W. Y.& Turcotte, Robert E.& Popovic, Snezana& Singh, Gurmit& Ghert, Michelle. AP-1 as a Regulator of MMP-13 in the Stromal Cell of Giant Cell Tumor of Bone. Biochemistry Research International. 2011. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-450991
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-450991