Characterising Cytokine Gene Expression Signatures in Patients with Severe Sepsis
Joint Authors
Kelleher, Dermot
Doherty, Derek G.
Ryan, Thomas
Grealy, Robert
White, Mary
McManus, Ross
Stordeur, Patrick
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-07-02
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Introduction.
Severe sepsis in humans may be related to an underlying profound immune suppressive state.
We investigated the link between gene expression of immune regulatory cytokines and the range of illness severity in patients with infection and severe sepsis.
Methods.
A prospective observational study included 54 ICU patients with severe sepsis, 53 patients with infection without organ failure, and 20 healthy controls.
Gene expression in peripheral blood mononuclear cells (PBMC) was measured using real-time polymerase chain reaction.
Results.
Infection differed from health by decreased expression of the IL2, and IL23 and greater expression of IL10 and IL27.
Severe sepsis differed from infection by having decreased IL7, IL23, IFNγ, and TNFα gene expression.
An algorithm utilising mRNA copy number for TNFα, IFNγ, IL7, IL10, and IL23 accurately distinguished sepsis from severe sepsis with a receiver operator characteristic value of 0.88.
Gene expression was similar with gram-positive and gram-negative infection and was similar following medical and surgical severe sepsis.
Severity of organ failure was associated with serum IL6 protein levels but not with any index of cytokine gene expression in PBMCs.
Conclusions.
Immune regulatory cytokine gene expression in PBMC provides a robust method of modelling patients’ response to infection.
American Psychological Association (APA)
Grealy, Robert& White, Mary& Stordeur, Patrick& Kelleher, Dermot& Doherty, Derek G.& McManus, Ross…[et al.]. 2013. Characterising Cytokine Gene Expression Signatures in Patients with Severe Sepsis. Mediators of Inflammation،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-450997
Modern Language Association (MLA)
Grealy, Robert…[et al.]. Characterising Cytokine Gene Expression Signatures in Patients with Severe Sepsis. Mediators of Inflammation No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-450997
American Medical Association (AMA)
Grealy, Robert& White, Mary& Stordeur, Patrick& Kelleher, Dermot& Doherty, Derek G.& McManus, Ross…[et al.]. Characterising Cytokine Gene Expression Signatures in Patients with Severe Sepsis. Mediators of Inflammation. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-450997
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-450997