Upregulation of Scavenger Receptor BI by Hepatic Nuclear Factor 4α through a Peroxisome Proliferator-Activated Receptor γ-Dependent Mechanism in Liver
Joint Authors
Xie, Xuefen
Zhang, Yi
Ai, Ding
Zhu, Yi
Shen, Chen
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-11-14
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Hepatic nuclear factor 4α (HNF4α) modulates the transcriptional activation of numerous metabolic genes in liver.
In this study, gene-array analysis revealed that HNF4α overexpression increased peroxisome proliferator-activated receptorγ (PPARγ) greatly in cultured rat primary hepatocytes.
PPAR-response-element-driven reporter gene expression could be elevated by HNF4α.
Bioinformatics analysis revealed a high-affinity HNF4α binding site in the human PPARγ2 promoter and in vitro experiments showed that this promoter could be transactivated by HNF4α.
The presence of HNF4α on the promoter was then confirmed by ChIP assay.
In vivo, hepatic overexpression of HNF4α decreased cholesterol levels both in plasma and liver and several hepatic genes related to cholesterol metabolism, including scavenger receptor BI (SR-BI), were upregulated.
The upregulation of SR-BI by HNF4α could be inhibited by a PPARγ antagonist in vitro.
In conclusion, HNF4α regulates cholesterol metabolism in rat by modulating the expression of SR-BI in the liver, in which the upregulation of PPARγ was involved.
American Psychological Association (APA)
Zhang, Yi& Shen, Chen& Ai, Ding& Xie, Xuefen& Zhu, Yi. 2011. Upregulation of Scavenger Receptor BI by Hepatic Nuclear Factor 4α through a Peroxisome Proliferator-Activated Receptor γ-Dependent Mechanism in Liver. PPAR Research،Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-451061
Modern Language Association (MLA)
Zhang, Yi…[et al.]. Upregulation of Scavenger Receptor BI by Hepatic Nuclear Factor 4α through a Peroxisome Proliferator-Activated Receptor γ-Dependent Mechanism in Liver. PPAR Research No. 2011 (2011), pp.1-10.
https://search.emarefa.net/detail/BIM-451061
American Medical Association (AMA)
Zhang, Yi& Shen, Chen& Ai, Ding& Xie, Xuefen& Zhu, Yi. Upregulation of Scavenger Receptor BI by Hepatic Nuclear Factor 4α through a Peroxisome Proliferator-Activated Receptor γ-Dependent Mechanism in Liver. PPAR Research. 2011. Vol. 2011, no. 2011, pp.1-10.
https://search.emarefa.net/detail/BIM-451061
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-451061