Vaccine Efficacy of Bm86 Ortholog of H. a. anatolicum, rHaa86 Expressed in Prokaryotic Expression System
Joint Authors
Ghosh, Srikanta
Gupta, Rohita
Chaudhuri, Pallab
Ray, D. D.
Azhahianambi, P.
Source
Journal of Parasitology Research
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-02-08
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The use of tick vaccine in controlling ticks and tick borne diseases has been proved effective in integrated tick management format.
For the control of H.
a.
anatolicum, Bm86 ortholog of H.
a.
anatolicum was cloned and expressed as fusion protein in E.
coli as E.
coli-pETHaa86.
The molecular weight of the rHaa86 was 97 kDa with a 19 kDa fusion tag of thioredoxin protein.
The expressed protein was characterized immunologically and vaccine efficacy was evaluated.
After 120 hours of challenge, only 26% tick could successfully fed on immunized animals.
Besides significant reduction in feeding percentages, a significant reduction of 49.6 mg; P<.01 in the weight of fed females in comparison to the females fed on control animals was recorded.
Following oviposition, a significant reduction of 68.1 mg; P<.05 in the egg masses of ticks fed on immunized animals in comparison to the ticks fed on control animals was noted.
The reduction of number of females, mean weight of eggs, adult females and efficacy of immunogen were 73.8%, 31.3%, 15.8%, and 82.3%, respectively.
The results indicated the possibility of development of rHaa86 based vaccine as a component of integrated control of tick species.
American Psychological Association (APA)
Azhahianambi, P.& Ray, D. D.& Chaudhuri, Pallab& Gupta, Rohita& Ghosh, Srikanta. 2010. Vaccine Efficacy of Bm86 Ortholog of H. a. anatolicum, rHaa86 Expressed in Prokaryotic Expression System. Journal of Parasitology Research،Vol. 2009, no. 2009, pp.1-7.
https://search.emarefa.net/detail/BIM-451144
Modern Language Association (MLA)
Azhahianambi, P.…[et al.]. Vaccine Efficacy of Bm86 Ortholog of H. a. anatolicum, rHaa86 Expressed in Prokaryotic Expression System. Journal of Parasitology Research No. 2009 (2009), pp.1-7.
https://search.emarefa.net/detail/BIM-451144
American Medical Association (AMA)
Azhahianambi, P.& Ray, D. D.& Chaudhuri, Pallab& Gupta, Rohita& Ghosh, Srikanta. Vaccine Efficacy of Bm86 Ortholog of H. a. anatolicum, rHaa86 Expressed in Prokaryotic Expression System. Journal of Parasitology Research. 2010. Vol. 2009, no. 2009, pp.1-7.
https://search.emarefa.net/detail/BIM-451144
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-451144