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Isolation of BAC Clones Containing Conserved Genes from Libraries of Three Distantly Related Moths : A Useful Resource for Comparative Genomics of Lepidoptera
Joint Authors
Kamimura, Manabu
Yasukochi, Yuji
Naito, Yota
Ishikawa, Yukio
Nakano, Ryo
Tanaka-Okuyama, Makiko
Sahara, Ken
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-11-25
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Lepidoptera, butterflies and moths, is the second largest animal order and includes numerous agricultural pests.
To facilitate comparative genomics in Lepidoptera, we isolated BAC clones containing conserved and putative single-copy genes from libraries of three pests, Heliothis virescens, Ostrinia nubilalis, and Plutella xylostella, harboring the haploid chromosome number, n=31, which are not closely related with each other or with the silkworm, Bombyx mori, (n=28), the sequenced model lepidopteran.
A total of 108–184 clones representing 101–182 conserved genes were isolated for each species.
For 79 genes, clones were isolated from more than two species, which will be useful as common markers for analysis using fluorescence in situ hybridization (FISH), as well as for comparison of genome sequence among multiple species.
The PCR-based clone isolation method presented here is applicable to species which lack a sequenced genome but have a significant collection of cDNA or EST sequences.
American Psychological Association (APA)
Yasukochi, Yuji& Tanaka-Okuyama, Makiko& Kamimura, Manabu& Nakano, Ryo& Naito, Yota& Ishikawa, Yukio…[et al.]. 2010. Isolation of BAC Clones Containing Conserved Genes from Libraries of Three Distantly Related Moths : A Useful Resource for Comparative Genomics of Lepidoptera. Journal of Biomedicine and Biotechnology،Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-451159
Modern Language Association (MLA)
Yasukochi, Yuji…[et al.]. Isolation of BAC Clones Containing Conserved Genes from Libraries of Three Distantly Related Moths : A Useful Resource for Comparative Genomics of Lepidoptera. Journal of Biomedicine and Biotechnology No. 2011 (2011), pp.1-6.
https://search.emarefa.net/detail/BIM-451159
American Medical Association (AMA)
Yasukochi, Yuji& Tanaka-Okuyama, Makiko& Kamimura, Manabu& Nakano, Ryo& Naito, Yota& Ishikawa, Yukio…[et al.]. Isolation of BAC Clones Containing Conserved Genes from Libraries of Three Distantly Related Moths : A Useful Resource for Comparative Genomics of Lepidoptera. Journal of Biomedicine and Biotechnology. 2010. Vol. 2011, no. 2011, pp.1-6.
https://search.emarefa.net/detail/BIM-451159
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-451159