Production and Partial Characterization of Cellulases from Trichoderma sp. IS-05 Isolated from Sandy Coastal Plains of Northeast Brazil
Joint Authors
Marbach, Phellippe Arthur Santos
Bispo, Aline Simões da Rocha
Andrade, Jackeline Pereira
do Nascimento, Rodrigo Pires
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-08-29
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
This study evaluated the production of cellulolytic enzymes by Trichoderma sp.
IS-05 strain, isolated from sand dunes, according to its ability to grow on cellulose as carbon source.
Wheat bran was tested as the carbon source and peptone tested as the nitrogen source.
Different concentrations of carbon and nitrogen were tested using a factorial design to identify optimal cellulase activity production.
The results showed that media containing wheat bran 4.0% (w/v) and peptone 0.25% (w/v) lead to the highest production, 564.0 U L−1 of cellulase, obtained after 2 days of fermentation.
The pH and temperature profile showed optimal activity at pH 3.0 and 60∘C.
As for thermostability, the cellulase was most tolerant at 60∘C, retaining more than 59.6% of maximal activity even after 4 hours of incubation.
The combination of acid pH, high temperature tolerance, and production of cellulase from agro-industrial residues by Trichoderma sp.
IS-05 offers possibilities condition for the biomass hydrolysis process to produce bioethanol.
American Psychological Association (APA)
Andrade, Jackeline Pereira& Bispo, Aline Simões da Rocha& Marbach, Phellippe Arthur Santos& do Nascimento, Rodrigo Pires. 2011. Production and Partial Characterization of Cellulases from Trichoderma sp. IS-05 Isolated from Sandy Coastal Plains of Northeast Brazil. Enzyme Research،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-451208
Modern Language Association (MLA)
Andrade, Jackeline Pereira…[et al.]. Production and Partial Characterization of Cellulases from Trichoderma sp. IS-05 Isolated from Sandy Coastal Plains of Northeast Brazil. Enzyme Research No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-451208
American Medical Association (AMA)
Andrade, Jackeline Pereira& Bispo, Aline Simões da Rocha& Marbach, Phellippe Arthur Santos& do Nascimento, Rodrigo Pires. Production and Partial Characterization of Cellulases from Trichoderma sp. IS-05 Isolated from Sandy Coastal Plains of Northeast Brazil. Enzyme Research. 2011. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-451208
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-451208