Red Ginseng Extract Facilitates the Early Differentiation of Human Embryonic Stem Cells into Mesendoderm Lineage
Joint Authors
Liu, Hung Ching
Choi, Young Min
Kim, Yoon Young
Moon, Shin Yong
Rosenwaks, Zev
Oh, Sun Kyung
Ku, Seung-Yup
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-09-01
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Human embryonic stem cells (hESCs) have capacities to self-renew and differentiate into all cell types in vitro.
Red ginseng (RG) is known to have a wide range of pharmacological effects in vivo; however, the reports on its effects on hESCs are few.
In this paper, we tried to demonstrate the effects of RG on the proliferation and differentiation of hESCs.
Undifferentiated hESCs, embryoid bodies (EBs), and hESC-derived cardiac progenitors (CPs) were treated with RG extract at 0.125, 0.25, and 0.5 mg/mL.
After treatment of undifferentiated hESCs from day 2 to day 6 of culture, BrdU labeling showed that RG treatment increased the proliferation of hESCs, and the expression of Oct4 and Nanog was increased in RG-treated group.
To find out the effects of RG on early differentiation stage cells, EBs were treated with RG extract for 10 days and attached for further differentiation.
Immunostaining for three germ layer markers showed that RG treatment increased the expressions of Brachyury and HNF3β on EBs.
Also, RG treatment increased the expression of Brachyury in early-stage and of Nkx2.5 in late-stage hESC-derived CPs.
These results demonstrate facilitating effects of RG extract on the proliferation and early differentiation of hESC.
American Psychological Association (APA)
Kim, Yoon Young& Ku, Seung-Yup& Rosenwaks, Zev& Liu, Hung Ching& Oh, Sun Kyung& Moon, Shin Yong…[et al.]. 2010. Red Ginseng Extract Facilitates the Early Differentiation of Human Embryonic Stem Cells into Mesendoderm Lineage. Evidence-Based Complementary and Alternative Medicine،Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-451219
Modern Language Association (MLA)
Kim, Yoon Young…[et al.]. Red Ginseng Extract Facilitates the Early Differentiation of Human Embryonic Stem Cells into Mesendoderm Lineage. Evidence-Based Complementary and Alternative Medicine No. 2011 (2011), pp.1-8.
https://search.emarefa.net/detail/BIM-451219
American Medical Association (AMA)
Kim, Yoon Young& Ku, Seung-Yup& Rosenwaks, Zev& Liu, Hung Ching& Oh, Sun Kyung& Moon, Shin Yong…[et al.]. Red Ginseng Extract Facilitates the Early Differentiation of Human Embryonic Stem Cells into Mesendoderm Lineage. Evidence-Based Complementary and Alternative Medicine. 2010. Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-451219
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-451219