Reduced PKC α Activity Induces Senescent Phenotype in Erythrocytes
Joint Authors
Govekar, Rukmini B.
Advani, Suresh H.
Kawle, Poonam D.
Zingde, Surekha M.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-09-04
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
The molecular mechanism mediating expression of senescent cell antigen-aggregated or cleaved band 3 and externalized phosphatidylserine (PS) on the surface of aged erythrocytes and their premature expression in certain anemias is not completely elucidated.
The erythrocytes with these surface modifications undergo macrophage-mediated phagocytosis.
In this study, the role of protein kinase C (PKC) isoforms in the expression of these surface modifications was investigated.
Inhibition of PKC α by 30 μM rottlerin (R30) and 2.3 nM Gö 6976 caused expression of both the senescent cell marker-externalized PS measured by FACS analysis and aggregated band 3 detected by western blotting.
In contrast to this observation, but in keeping with literature, PKC activation by phorbol-12-myristate-13-acetate (PMA) also led to the expression of senescence markers.
We explain this antithesis by demonstrating that PMA-treated cells show reduction in the activity of PKC α, thereby simulating inhibition.
The reduction in PKC α activity may be attributed to the known downregulation of PMA-activated PKC α, caused by its membrane translocation and proteolysis.
We demonstrate membrane translocation of PKC α in PMA-treated cells to substantiate this inference.
Thus loss of PKC α activity either by inhibition or downregulation can cause surface modifications which can trigger erythrophagocytosis.
American Psychological Association (APA)
Govekar, Rukmini B.& Kawle, Poonam D.& Advani, Suresh H.& Zingde, Surekha M.. 2012. Reduced PKC α Activity Induces Senescent Phenotype in Erythrocytes. Anemia،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-451280
Modern Language Association (MLA)
Govekar, Rukmini B.…[et al.]. Reduced PKC α Activity Induces Senescent Phenotype in Erythrocytes. Anemia No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-451280
American Medical Association (AMA)
Govekar, Rukmini B.& Kawle, Poonam D.& Advani, Suresh H.& Zingde, Surekha M.. Reduced PKC α Activity Induces Senescent Phenotype in Erythrocytes. Anemia. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-451280
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-451280