A Protocol for Measurement of Noncoding RNA in Human Serum
Joint Authors
Satoor, Sarang N.
Joglekar, Mugdha V.
Taylor, Caroline J.
Pereira e Cotta, Maria V.
Ranjan, Amaresh K.
Source
Experimental Diabetes Research
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-4, 4 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-01
Country of Publication
Egypt
No. of Pages
4
Main Subjects
Abstract EN
MicroRNAs (miRNAs) are small noncoding RNAs that act as regulators of gene expression by targeting mature messenger RNAs.
Following the initial report of the presence of miRNAs in serum and plasma a number of studies have successfully demonstrated the use of these miRNAs as biomarkers of disease.
Currently, there are many methods of isolating total RNA from liquid samples.
Here, we describe a simple, cost effective method for extraction of RNA from human serum as well as subsequent real time PCR analysis of miRNA levels.
American Psychological Association (APA)
Taylor, Caroline J.& Satoor, Sarang N.& Ranjan, Amaresh K.& Pereira e Cotta, Maria V.& Joglekar, Mugdha V.. 2012. A Protocol for Measurement of Noncoding RNA in Human Serum. Experimental Diabetes Research،Vol. 2012, no. 2012, pp.1-4.
https://search.emarefa.net/detail/BIM-451313
Modern Language Association (MLA)
Taylor, Caroline J.…[et al.]. A Protocol for Measurement of Noncoding RNA in Human Serum. Experimental Diabetes Research No. 2012 (2012), pp.1-4.
https://search.emarefa.net/detail/BIM-451313
American Medical Association (AMA)
Taylor, Caroline J.& Satoor, Sarang N.& Ranjan, Amaresh K.& Pereira e Cotta, Maria V.& Joglekar, Mugdha V.. A Protocol for Measurement of Noncoding RNA in Human Serum. Experimental Diabetes Research. 2012. Vol. 2012, no. 2012, pp.1-4.
https://search.emarefa.net/detail/BIM-451313
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-451313
