Upregulation of Endogenous HMOX1 Expression by a Computer-Designed Artificial Transcription Factor
Joint Authors
Wei, Yong
Lu, Hai
Tian, Yi
Guo, Hongfeng
Ying, Dajun
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-07-14
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Heme oxygenase-1 (HO-1) is well known as a cytoprotective factor.
Research has revealed that it is a promising therapeutic target for cardiovascular diseases.
In the current study, an HMOX1 (HO-1 gene) enhancer-specific artificial zinc-finger protein (AZP) was designed using bioinformatical methods.
Then, an artificial transcription factor (ATF) was constructed based on the AZP.
In the ATF, the p65 functional domain was used as the effector domain (ED), and a nuclear localization sequence (NLS) was also included.
We next analyzed the affinity of the ATF to the HMOX1 enhancer and the effect of the ATF on endogenous HMOX1 expression.
The results suggest that the ATF could effectively upregulate endogenous HMOX1 expression in ECV304 cells.
With further research, the ATF could be developed as a potential drug for cardiovascular diseases.
American Psychological Association (APA)
Guo, Hongfeng& Tian, Yi& Lu, Hai& Wei, Yong& Ying, Dajun. 2010. Upregulation of Endogenous HMOX1 Expression by a Computer-Designed Artificial Transcription Factor. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-451344
Modern Language Association (MLA)
Guo, Hongfeng…[et al.]. Upregulation of Endogenous HMOX1 Expression by a Computer-Designed Artificial Transcription Factor. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-7.
https://search.emarefa.net/detail/BIM-451344
American Medical Association (AMA)
Guo, Hongfeng& Tian, Yi& Lu, Hai& Wei, Yong& Ying, Dajun. Upregulation of Endogenous HMOX1 Expression by a Computer-Designed Artificial Transcription Factor. Journal of Biomedicine and Biotechnology. 2010. Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-451344
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-451344