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Trichostatin A Suppresses EGFR Expression through Induction of MicroRNA-7 in an HDAC-Independent Manner in Lapatinib-Treated Cells
Joint Authors
Yu, Meng-Chieh
Liu, Liang-Chih
Hsia, Te-Chun
Huang, Wei-Chien
Chen, Chia-Hung
Chien, Pei-Hsuan
Hsu, Ke-Wei
Wei, Ya-Ling
Yeh, Ming-Hsin
Chen, Wen-Shu
Tu, Chih-Yen
Hsu, Min-Hsiang
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-02-22
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Lapatinib, a dual EGFR/HER2 tyrosine kinase inhibitor, has been shown to improve the survival rate of patients with advanced HER2-positive breast cancers.
However, the off-target activity of lapatinib in inducing EGFR expression without tyrosine kinase activity was demonstrated to render HER2-negative breast cancer cells more metastatic, suggesting a limitation to the therapeutic effectiveness of this dual inhibitor in HER2-heterogeneous tumors.
Therefore, targeting EGFR expression may be a feasible approach to improve the anticancer efficiency of lapatinib-based therapy.
Inhibition of HDAC has been previously reported to epigenetically suppress EGFR protein expression.
In this study, however, our data indicated that treatment with HDAC inhibitors trichostatin A (TSA), but not suberoylanilide hydroxamic acid (SAHA) or HDAC siRNA, can attenuate both protein and mRNA expressions of EGFR in lapatinib-treated triple-negative breast cancer cells, suggesting that TSA may suppress EGFR expression independently of HDAC inhibition.
Nevertheless, TSA reduced EGFR 3′UTR activity and induced the gene expression of microRNA-7, a known EGFR-targeting microRNA.
Furthermore, treatment with microRNA-7 inhibitor attenuated TSA-mediated EGFR suppression.
These results suggest that TSA induced microRNA-7 expression to downregulate EGFR expression in an HDAC-independent manner.
American Psychological Association (APA)
Tu, Chih-Yen& Chen, Chia-Hung& Hsia, Te-Chun& Hsu, Min-Hsiang& Wei, Ya-Ling& Yu, Meng-Chieh…[et al.]. 2014. Trichostatin A Suppresses EGFR Expression through Induction of MicroRNA-7 in an HDAC-Independent Manner in Lapatinib-Treated Cells. BioMed Research International،Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-451366
Modern Language Association (MLA)
Tu, Chih-Yen…[et al.]. Trichostatin A Suppresses EGFR Expression through Induction of MicroRNA-7 in an HDAC-Independent Manner in Lapatinib-Treated Cells. BioMed Research International No. 2014 (2014), pp.1-11.
https://search.emarefa.net/detail/BIM-451366
American Medical Association (AMA)
Tu, Chih-Yen& Chen, Chia-Hung& Hsia, Te-Chun& Hsu, Min-Hsiang& Wei, Ya-Ling& Yu, Meng-Chieh…[et al.]. Trichostatin A Suppresses EGFR Expression through Induction of MicroRNA-7 in an HDAC-Independent Manner in Lapatinib-Treated Cells. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-11.
https://search.emarefa.net/detail/BIM-451366
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-451366