Comparison of Extraction Methods of Chitin from Ganoderma lucidum Mushroom Obtained in Submerged Culture
Joint Authors
Rojas Vahos, Diego Fernando
Ospina Álvarez, Sandra Patricia
Atehortúa, Lucía
Escobar Sierra, Diana Marcela
Zapata Ocampo, Paola
Ossa Orozco, Claudia Patricia
Ramírez Cadavid, David Alexander
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-01-15
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The chitin was isolated from the Ganoderma lucidum submerged cultures mycelium as potential source of chitin under biotechnological processes.
The extraction of chitin was carried out through 5 different assays which involved mainly three phases: pulverization of the mushroom, deproteinization of the mycelia with NaOH solution, and a process of decolorization with potassium permanganate and oxalic acid.
The chitin contents extracted from 9-day mycelia were 413, 339, 87, 78, and 144 mg/g−1 (milligrams of chitin/grams of dry biomass) for A1, A2, A3, A4, and A5, respectively.
Obtained chitin was characterized by X-Ray Diffraction (XRD), by Fourier transform infrared spectroscopy (FTIR), and by thermal analysis (TGA).
The results showed that Ganoderma lucidum chitin has similar characteristic of chitin from different fonts.
The advantage of the biotechnological processes and the fact that Ganoderma lucidum fungus may be used as a potential raw material for chitin production were demonstrated.
American Psychological Association (APA)
Ospina Álvarez, Sandra Patricia& Ramírez Cadavid, David Alexander& Escobar Sierra, Diana Marcela& Ossa Orozco, Claudia Patricia& Rojas Vahos, Diego Fernando& Zapata Ocampo, Paola…[et al.]. 2014. Comparison of Extraction Methods of Chitin from Ganoderma lucidum Mushroom Obtained in Submerged Culture. BioMed Research International،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-451379
Modern Language Association (MLA)
Ospina Álvarez, Sandra Patricia…[et al.]. Comparison of Extraction Methods of Chitin from Ganoderma lucidum Mushroom Obtained in Submerged Culture. BioMed Research International No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-451379
American Medical Association (AMA)
Ospina Álvarez, Sandra Patricia& Ramírez Cadavid, David Alexander& Escobar Sierra, Diana Marcela& Ossa Orozco, Claudia Patricia& Rojas Vahos, Diego Fernando& Zapata Ocampo, Paola…[et al.]. Comparison of Extraction Methods of Chitin from Ganoderma lucidum Mushroom Obtained in Submerged Culture. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-451379
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-451379