Overexpression of D-Xylose Reductase (xyl1) Gene and Antisense Inhibition of D-Xylulokinase (xyiH) Gene Increase Xylitol Production in Trichoderma reesei
Joint Authors
Kepka, Greg
Dashtban, Mehdi
Chen, Sanfeng
Qin, Wensheng
Hong, Yuanyuan
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-11
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
T.
reesei is an efficient cellulase producer and biomass degrader.
To improve xylitol production in Trichoderma reesei strains by genetic engineering, two approaches were used in this study.
First, the presumptive D-xylulokinase gene in T.
reesei (xyiH), which has high homology to known fungi D-xylulokinase genes, was silenced by transformation of T.
reesei QM9414 strain with an antisense construct to create strain S6-2-2.
The expression of the xyiH gene in the transformed strain S6-2-2 decreased at the mRNA level, and D-xylulokinase activity decreased after 48 h of incubation.
This led to an increase in xylitol production from undetectable levels in wild-type T.
reesei QM9414 to 8.6 mM in S6-2-2.
The T.
reesei Δxdh is a xylose dehydrogenase knockout strain with increased xylitol production compared to the wild-type T.
reesei QM9414 (22.8 mM versus undetectable).
The copy number of the xylose reductase gene (xyl1) in T.
reesei Δxdh strain was increased by genetic engineering to create a new strain Δ9-5-1.
The Δ9-5-1 strain showed a higher xyl1 expression and a higher yield of xylose reductase, and xylitol production was increased from 22.8 mM to 24.8 mM.
Two novel strains S6-2-2 and Δ9-5-1 are capable of producing higher yields of xylitol.
T.
reesei has great potential in the industrial production of xylitol.
American Psychological Association (APA)
Hong, Yuanyuan& Dashtban, Mehdi& Kepka, Greg& Chen, Sanfeng& Qin, Wensheng. 2014. Overexpression of D-Xylose Reductase (xyl1) Gene and Antisense Inhibition of D-Xylulokinase (xyiH) Gene Increase Xylitol Production in Trichoderma reesei. BioMed Research International،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-451436
Modern Language Association (MLA)
Hong, Yuanyuan…[et al.]. Overexpression of D-Xylose Reductase (xyl1) Gene and Antisense Inhibition of D-Xylulokinase (xyiH) Gene Increase Xylitol Production in Trichoderma reesei. BioMed Research International No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-451436
American Medical Association (AMA)
Hong, Yuanyuan& Dashtban, Mehdi& Kepka, Greg& Chen, Sanfeng& Qin, Wensheng. Overexpression of D-Xylose Reductase (xyl1) Gene and Antisense Inhibition of D-Xylulokinase (xyiH) Gene Increase Xylitol Production in Trichoderma reesei. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-451436
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-451436