Intron Retention and TE Exonization Events in ZRANB2
Joint Authors
Huh, Jae-Won
Chang, Kyu-Tae
Kim, Heui-Soo
Kim, Young-Hyun
Park, Sang-Je
Source
Comparative and Functional Genomics
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-06-17
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The Zinc finger, RAN-binding domain-containing protein 2 (ZRANB2), contains arginine/serine-rich (RS) domains that mediate its function in the regulation of alternative splicing.
The ZRANB2 gene contains 2 LINE elements (L3b, Plat_L3) between the 9th and 10th exons.
We identified the exonization event of a LINE element (Plat_L3).
Using genomic PCR, RT-PCR amplification, and sequencing of primate DNA and RNA samples, we analyzed the evolutionary features of ZRANB2 transcripts.
The results indicated that 2 of the LINE elements were integrated in human and all of the tested primate samples (hominoids: 3 species; Old World monkey: 8 species; New World monkey: 6 species; prosimian: 1 species).
Human, rhesus monkey, crab-eating monkey, African-green monkey, and marmoset harbor the exon derived from LINE element (Plat_L3).
RT-PCR amplification revealed the long transcripts and their differential expression patterns.
Intriguingly, these long transcripts were abundantly expressed in Old World monkey lineages (rhesus, crab-eating, and African-green monkeys) and were expressed via intron retention (IR).
Thus, the ZRANB2 gene produces 3 transcript variants in which the Cterminus varies by transposable elements (TEs) exonization and IR mechanisms.
Therefore, ZRANB2 is valuable for investigating the evolutionary mechanisms of TE exonization and IR during primate evolution.
American Psychological Association (APA)
Park, Sang-Je& Huh, Jae-Won& Kim, Young-Hyun& Kim, Heui-Soo& Chang, Kyu-Tae. 2012. Intron Retention and TE Exonization Events in ZRANB2. Comparative and Functional Genomics،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-451461
Modern Language Association (MLA)
Park, Sang-Je…[et al.]. Intron Retention and TE Exonization Events in ZRANB2. Comparative and Functional Genomics No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-451461
American Medical Association (AMA)
Park, Sang-Je& Huh, Jae-Won& Kim, Young-Hyun& Kim, Heui-Soo& Chang, Kyu-Tae. Intron Retention and TE Exonization Events in ZRANB2. Comparative and Functional Genomics. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-451461
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-451461