Intron Retention and TE Exonization Events in ZRANB2

Joint Authors

Huh, Jae-Won
Chang, Kyu-Tae
Kim, Heui-Soo
Kim, Young-Hyun
Park, Sang-Je

Source

Comparative and Functional Genomics

Issue

Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2012-06-17

Country of Publication

Egypt

No. of Pages

7

Main Subjects

Biology

Abstract EN

The Zinc finger, RAN-binding domain-containing protein 2 (ZRANB2), contains arginine/serine-rich (RS) domains that mediate its function in the regulation of alternative splicing.

The ZRANB2 gene contains 2 LINE elements (L3b, Plat_L3) between the 9th and 10th exons.

We identified the exonization event of a LINE element (Plat_L3).

Using genomic PCR, RT-PCR amplification, and sequencing of primate DNA and RNA samples, we analyzed the evolutionary features of ZRANB2 transcripts.

The results indicated that 2 of the LINE elements were integrated in human and all of the tested primate samples (hominoids: 3 species; Old World monkey: 8 species; New World monkey: 6 species; prosimian: 1 species).

Human, rhesus monkey, crab-eating monkey, African-green monkey, and marmoset harbor the exon derived from LINE element (Plat_L3).

RT-PCR amplification revealed the long transcripts and their differential expression patterns.

Intriguingly, these long transcripts were abundantly expressed in Old World monkey lineages (rhesus, crab-eating, and African-green monkeys) and were expressed via intron retention (IR).

Thus, the ZRANB2 gene produces 3 transcript variants in which the Cterminus varies by transposable elements (TEs) exonization and IR mechanisms.

Therefore, ZRANB2 is valuable for investigating the evolutionary mechanisms of TE exonization and IR during primate evolution.

American Psychological Association (APA)

Park, Sang-Je& Huh, Jae-Won& Kim, Young-Hyun& Kim, Heui-Soo& Chang, Kyu-Tae. 2012. Intron Retention and TE Exonization Events in ZRANB2. Comparative and Functional Genomics،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-451461

Modern Language Association (MLA)

Park, Sang-Je…[et al.]. Intron Retention and TE Exonization Events in ZRANB2. Comparative and Functional Genomics No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-451461

American Medical Association (AMA)

Park, Sang-Je& Huh, Jae-Won& Kim, Young-Hyun& Kim, Heui-Soo& Chang, Kyu-Tae. Intron Retention and TE Exonization Events in ZRANB2. Comparative and Functional Genomics. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-451461

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-451461