Cyclohexylmethyl Flavonoids Suppress Propagation of Breast Cancer Stem Cells via Downregulation of NANOG
Joint Authors
Kuo, Sheng-Chu
Shen, Chia-Ning
Hwang, Shiaw-Min
Hsu, Hung-Wei
Liao, Wen-Ying
Han, Hsin-Ying
Huang, Yuan-Chao
Liaw, Chih-Chuang
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-04-04
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Breast cancer stem cells (CSCs) are highly tumorigenic and possess the capacity to self-renew.
Recent studies indicated that pluripotent gene NANOG involves in regulating self-renewal of breast CSCs, and expression of NANOG is correlated with aggressiveness of poorly differentiated breast cancer.
We initially confirmed that breast cancer MCF-7 cells expressed NANOG, and overexpression of NANOG enhanced the tumorigenicity of MCF-7 cells and promoted the self-renewal expansion of CD24−/lowCD44+ CSC subpopulation.
In contrast, knockdown of NANOG significantly affected the growth of breast CSCs.
Utilizing flow cytometry, we identified five cyclohexylmethyl flavonoids that can inhibit propagation of NANOG-positive cells in both breast cancer MCF-7 and MDA-MB231 cells.
Among these flavonoids, ugonins J and K were found to be able to induce apoptosis in non-CSC populations and to reduce self-renewal growth of CD24−/lowCD44+ CSC population.
Treatment with ugonin J significantly reduced the tumorigenicity of MCF-7 cells and efficiently suppressed formation of mammospheres.
This suppression was possibly due to p53 activation and NANOG reduction as either addition of p53 inhibitor or overexpression of NANOG can counteract the suppressive effect of ugonin J.
We therefore conclude that cyclohexylmethyl flavonoids can possibly be utilized to suppress the propagation of breast CSCs via reduction of NANOG.
American Psychological Association (APA)
Liao, Wen-Ying& Liaw, Chih-Chuang& Huang, Yuan-Chao& Han, Hsin-Ying& Hsu, Hung-Wei& Hwang, Shiaw-Min…[et al.]. 2013. Cyclohexylmethyl Flavonoids Suppress Propagation of Breast Cancer Stem Cells via Downregulation of NANOG. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-14.
https://search.emarefa.net/detail/BIM-451467
Modern Language Association (MLA)
Liao, Wen-Ying…[et al.]. Cyclohexylmethyl Flavonoids Suppress Propagation of Breast Cancer Stem Cells via Downregulation of NANOG. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-14.
https://search.emarefa.net/detail/BIM-451467
American Medical Association (AMA)
Liao, Wen-Ying& Liaw, Chih-Chuang& Huang, Yuan-Chao& Han, Hsin-Ying& Hsu, Hung-Wei& Hwang, Shiaw-Min…[et al.]. Cyclohexylmethyl Flavonoids Suppress Propagation of Breast Cancer Stem Cells via Downregulation of NANOG. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-14.
https://search.emarefa.net/detail/BIM-451467
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-451467