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Clinical Evaluation of COBAS TaqMan PCR for the Detection of Mycobacterium tuberculosis and M. avium Complex
Joint Authors
Kajiki, Akira
Sakoda, Yoritake
Kawasaki, Masayuki
Ikegame, Satoshi
Taguchi, Kazuhito
Fujino, Nao
Source
Tuberculosis Research and Treatment
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-09-17
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
A retrospective observational study was performed to determine the sensitivity and limitation of PCR test for the detection of Mycobacterium tuberculosis and M.
avium complex.
We obtained clinical specimens collected from the respiratory tract, cultured M.
tuberculosis or M.
avium complex, and performed PCR analysis.
A total of 299 samples (M.
tuberculosis, 177; M.
avium, 35; M.
intracellulare, 87) were analyzed by COBAS TaqMan PCR from April 2007 to March 2011.
The PCR positivity rates were 50–55%, 70–100%, 88–98%, and 100% in smear-negative, smear 1+, 2+, and 3+ groups, respectively.
The PCR positivity of tuberculosis in smear 1+ was 80.6%, which was statistically significantly (P<0.001) lower than that of smear 2+ (97.3%).
From January 2005 to March 2007, we collected an additional 138 samples (M.
tuberculosis, 74; M.
avium, 21; M.
intracellulare, 43), which were analyzed by COBAS Amplicor PCR.
The PCR positivity rates obtained using COBAS TaqMan PCR and COBAS Amplicor PCR were not significantly different.
The sensitivity of PCR test for mycobacteria is not sufficient in case of smear 1+.
Careful consideration must be given to the interpretation of negative PCR test results in smear 1+, because smear-positive tuberculosis is the criterion for isolation.
American Psychological Association (APA)
Ikegame, Satoshi& Sakoda, Yoritake& Fujino, Nao& Taguchi, Kazuhito& Kawasaki, Masayuki& Kajiki, Akira. 2012. Clinical Evaluation of COBAS TaqMan PCR for the Detection of Mycobacterium tuberculosis and M. avium Complex. Tuberculosis Research and Treatment،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-451488
Modern Language Association (MLA)
Ikegame, Satoshi…[et al.]. Clinical Evaluation of COBAS TaqMan PCR for the Detection of Mycobacterium tuberculosis and M. avium Complex. Tuberculosis Research and Treatment No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-451488
American Medical Association (AMA)
Ikegame, Satoshi& Sakoda, Yoritake& Fujino, Nao& Taguchi, Kazuhito& Kawasaki, Masayuki& Kajiki, Akira. Clinical Evaluation of COBAS TaqMan PCR for the Detection of Mycobacterium tuberculosis and M. avium Complex. Tuberculosis Research and Treatment. 2012. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-451488
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-451488