![](/images/graphics-bg.png)
Receptor Binding by Cholera Toxin B-Subunit and Amino Acid Modification Improves Minimal Peptide Immunogenicity
Joint Authors
Boberg, Andreas
Wahren, Britta
Carlin, Nils
Stålnacke, Alexandra
Hinkula, Jorma
Bråve, Andreas
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-15
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Abstract EN
We increase our understanding of augmenting a cellular immune response, by using an HIV-1 protease-derived epitope (PR75–84), and variants thereof, coupled to the C-terminal, of the B subunit of cholera toxin (CTB).
Fusion proteins were used for immunizations of HLA-A0201 transgenic C57BL/6 mice.
We observed different capacities to elicit a cellular immune response by peptides with additions of five to ten amino acids to the PR epitope.
There was a positive correlation between the magnitude of the elicited cellular immune response and the capacity of the fusion protein to bind GM-1.
This binding capacity is affected by its ability to form natural pentamers of CTB.
Our results suggest that functional CTB pentamers containing a foreign amino acid-modified epitope is a novel way to overcome the limited cellular immunogenicity of minimal peptide antigens.
This way of using a functional assay as readout for improved cellular immunogenicity might become highly valuable for difficult immunogens such as short peptides (epitopes).
American Psychological Association (APA)
Boberg, Andreas& Stålnacke, Alexandra& Bråve, Andreas& Hinkula, Jorma& Wahren, Britta& Carlin, Nils. 2012. Receptor Binding by Cholera Toxin B-Subunit and Amino Acid Modification Improves Minimal Peptide Immunogenicity. ISRN Molecular Biology،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-451507
Modern Language Association (MLA)
Boberg, Andreas…[et al.]. Receptor Binding by Cholera Toxin B-Subunit and Amino Acid Modification Improves Minimal Peptide Immunogenicity. ISRN Molecular Biology No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-451507
American Medical Association (AMA)
Boberg, Andreas& Stålnacke, Alexandra& Bråve, Andreas& Hinkula, Jorma& Wahren, Britta& Carlin, Nils. Receptor Binding by Cholera Toxin B-Subunit and Amino Acid Modification Improves Minimal Peptide Immunogenicity. ISRN Molecular Biology. 2012. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-451507
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-451507