Rapamycin Conditioning of Dendritic Cells Differentiated from Human ES Cells Promotes a Tolerogenic Phenotype
Joint Authors
Nishimoto, Kevin P.
Leishman, Alison J.
Silk, Kathryn M.
Reddy, Anita
Fairchild, Paul J.
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-02-07
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
While human embryonic stem cells (hESCs) may one day facilitate the treatment of degenerative diseases requiring cell replacement therapy, the success of regenerative medicine is predicated on overcoming the rejection of replacement tissues.
Given the role played by dendritic cells (DCs) in the establishment of immunological tolerance, we have proposed that DC, rendered tolerogenic during their differentiation from hESC, might predispose recipients to accept replacement tissues.
As a first step towards this goal, we demonstrate that DC differentiated from H1 hESCs (H1-DCs) are particularly responsive to the immunosuppressive agent rapamycin compared to monocyte-derived DC (moDC).
While rapamycin had only modest impact on the phenotype and function of moDC, H1-DC failed to upregulate CD40 upon maturation and displayed reduced immunostimulatory capacity.
Furthermore, coculture of naïve allogeneic T cells with rapamycin-treated H1-DC promoted an increased appearance of CD25hi Foxp3+ regulatory T cells, compared to moDC.
Our findings suggest that conditioning of hESC-derived DC with rapamycin favours a tolerogenic phenotype.
American Psychological Association (APA)
Silk, Kathryn M.& Leishman, Alison J.& Nishimoto, Kevin P.& Reddy, Anita& Fairchild, Paul J.. 2012. Rapamycin Conditioning of Dendritic Cells Differentiated from Human ES Cells Promotes a Tolerogenic Phenotype. Journal of Biomedicine and Biotechnology،Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-451654
Modern Language Association (MLA)
Silk, Kathryn M.…[et al.]. Rapamycin Conditioning of Dendritic Cells Differentiated from Human ES Cells Promotes a Tolerogenic Phenotype. Journal of Biomedicine and Biotechnology No. 2012 (2012), pp.1-11.
https://search.emarefa.net/detail/BIM-451654
American Medical Association (AMA)
Silk, Kathryn M.& Leishman, Alison J.& Nishimoto, Kevin P.& Reddy, Anita& Fairchild, Paul J.. Rapamycin Conditioning of Dendritic Cells Differentiated from Human ES Cells Promotes a Tolerogenic Phenotype. Journal of Biomedicine and Biotechnology. 2012. Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-451654
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-451654