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Combined Cytogenotoxic Effects of Bee Venom and Bleomycin on Rat Lymphocytes : An In Vitro Study
Joint Authors
Awad, Ashraf
Khalil, Samah R.
Abd-Elhakim, Yasmina M.
al-Ayadhi, Laila Y.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-16
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
This study was carried out to determine the cytotoxic and genotoxic effects of bee venom (BV) and/or the chemotherapeutic agent bleomycin (BLM) on healthy isolated rat lymphocytes utilizing morphometric and molecular techniques.
Using the Ficoll-Histopaque density gradient centrifugation technique, lymphocytes were isolated, divided into groups, and subjected to BV and/or BLM at incubation medium concentrations of 10 or 20 μg/mL respectively for 24 and 72 hrs.
An MTT assay and fluorescent microscopy examinations were used to assess the cytotoxic effects.
To determine the predominant type of BV and/or BLM-induced cell death, LDH release assay was employed beside quantitative expression analyses of the apoptosis-related genes (Caspase-3 and Bcl-2).
The genotoxic effects of the tested compounds were evaluated via DNA fragmentation assay.
The results of these assays demonstrated that BV potentiates BLM-induced cytotoxicity through increased LDH release and diminished cell viability.
Nevertheless, BV significantly inhibited the BLM-induced DNA damage.
The results verify that BV significantly attenuates the genotoxic effects of BLM on noncancerous isolated rat lymphocytes but does not diminish BLM cytotoxicity.
American Psychological Association (APA)
Abd-Elhakim, Yasmina M.& Khalil, Samah R.& Awad, Ashraf& al-Ayadhi, Laila Y.. 2014. Combined Cytogenotoxic Effects of Bee Venom and Bleomycin on Rat Lymphocytes : An In Vitro Study. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-451798
Modern Language Association (MLA)
Abd-Elhakim, Yasmina M.…[et al.]. Combined Cytogenotoxic Effects of Bee Venom and Bleomycin on Rat Lymphocytes : An In Vitro Study. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-451798
American Medical Association (AMA)
Abd-Elhakim, Yasmina M.& Khalil, Samah R.& Awad, Ashraf& al-Ayadhi, Laila Y.. Combined Cytogenotoxic Effects of Bee Venom and Bleomycin on Rat Lymphocytes : An In Vitro Study. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-451798
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-451798