Attenuation of Myocardial Injury by HMGB1 Blockade during IschemiaReperfusion Is Toll-Like Receptor 2-Dependent
Joint Authors
Sprunck, Vera
Latsch, Kathrina
Zacharowski, Kai
Mersmann, Jan
Iskandar, Franziska
Koch, Alexander
Schumann, Ralf R.
Zimmermann, René
Habeck, Katharina
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-11-24
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Genetic or pharmacological ablation of toll-like receptor 2 (TLR2) protects against myocardial ischemia/reperfusion injury (MI/R).
However, the endogenous ligand responsible for TLR2 activation has not yet been detected.
The objective of this study was to identify HMGB1 as an activator of TLR2 signalling during MI/R.
C57BL/6 wild-type (WT) or TLR2−/−-mice were injected with vehicle, HMGB1, or HMGB1 BoxA one hour before myocardial ischemia (30 min) and reperfusion (24 hrs).
Infarct size, cardiac troponin T, leukocyte infiltration, HMGB1 release, TLR4-, TLR9-, and RAGE-expression were quantified.
HMGB1 plasma levels were measured in patients undergoing coronary artery bypass graft (CABG) surgery.
HMGB1 antagonist BoxA reduced cardiomyocyte necrosis during MI/R in WT mice, accompanied by reduced leukocyte infiltration.
Injection of HMGB1 did, however, not increase infarct size in WT animals.
In TLR2−/−-hearts, neither BoxA nor HMGB1 affected infarct size.
No differences in RAGE and TLR9 expression could be detected, while TLR2−/−-mice display increased TLR4 and HMGB1 expression.
Plasma levels of HMGB1 were increased MI/R in TLR2−/−-mice after CABG surgery in patients carrying a TLR2 polymorphism (Arg753Gln).
We here provide evidence that absence of TLR2 signalling abrogates infarct-sparing effects of HMGB1 blockade.
American Psychological Association (APA)
Mersmann, Jan& Iskandar, Franziska& Latsch, Kathrina& Habeck, Katharina& Sprunck, Vera& Zimmermann, René…[et al.]. 2013. Attenuation of Myocardial Injury by HMGB1 Blockade during IschemiaReperfusion Is Toll-Like Receptor 2-Dependent. Mediators of Inflammation،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-451825
Modern Language Association (MLA)
Mersmann, Jan…[et al.]. Attenuation of Myocardial Injury by HMGB1 Blockade during IschemiaReperfusion Is Toll-Like Receptor 2-Dependent. Mediators of Inflammation No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-451825
American Medical Association (AMA)
Mersmann, Jan& Iskandar, Franziska& Latsch, Kathrina& Habeck, Katharina& Sprunck, Vera& Zimmermann, René…[et al.]. Attenuation of Myocardial Injury by HMGB1 Blockade during IschemiaReperfusion Is Toll-Like Receptor 2-Dependent. Mediators of Inflammation. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-451825
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-451825