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Benidipine Protects Kidney through Inhibiting ROCK1 Activity and Reducing the Epithelium-Mesenchymal Transdifferentiation in Type 1 Diabetic Rats
Joint Authors
Xu, Kui
Wu, Ganlin
Hu, Yilan
Xu, Meirong
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-01
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
We investigated the protective effect of benidipine, by testing the changes of the activity of Rho kinase and transdifferentiation of renal tubular epithelium cells in vivo.
Wistar rats were randomly divided into two groups: normal (N) and diabetes.
STZ were used to make the rats type 1 diabetic and were randomly assigned as diabetes without treatment (D), diabetes treated with benidipine (B), and diabetes treated with fasudil (F) and treated for 3 months.
Immunohistochemistry and western blotting were for protein expressions of ROCK1, α-SMA, and E-cadherin and real-time PCR for the mRNA quantification of ROCK1.
Compared with N group, D group had significant proliferation of glomerular mesangial matrix, increased cell number, thickened basement membrane, widely infiltrated by inflammatory cells and fibrosis in the renal interstitial, and dilated tubular.
Those presentations in F and B groups were milder.
Compared with N group, D group showed elevated MYPT1 phosphorylation, increased expression of ROCK1, α-SMA protein, and ROCK1 mRNA and decreased expression of E-cadherin protein.
B group showed attenuated MYPT1 phosphorylation, decreased ROCK1, α-SMA protein, and ROCK1 mRNA expression and increased expression of E-cadherin protein.
In conclusion, benidipine reduces the epithelium-mesenchymal transdifferentiation and renal interstitial fibrosis in diabetic kidney by inhibiting ROCK1 activity.
American Psychological Association (APA)
Wu, Ganlin& Xu, Meirong& Xu, Kui& Hu, Yilan. 2013. Benidipine Protects Kidney through Inhibiting ROCK1 Activity and Reducing the Epithelium-Mesenchymal Transdifferentiation in Type 1 Diabetic Rats. Journal of Diabetes Research،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-451849
Modern Language Association (MLA)
Wu, Ganlin…[et al.]. Benidipine Protects Kidney through Inhibiting ROCK1 Activity and Reducing the Epithelium-Mesenchymal Transdifferentiation in Type 1 Diabetic Rats. Journal of Diabetes Research No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-451849
American Medical Association (AMA)
Wu, Ganlin& Xu, Meirong& Xu, Kui& Hu, Yilan. Benidipine Protects Kidney through Inhibiting ROCK1 Activity and Reducing the Epithelium-Mesenchymal Transdifferentiation in Type 1 Diabetic Rats. Journal of Diabetes Research. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-451849
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-451849