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A Novel Pathway for the Biosynthesis of Heme in Archaea : Genome-Based Bioinformatic Predictions and Experimental Evidence
Joint Authors
Raux-Deery, Evelyne
Warren, Martin J.
Rolfes, Sarah
Jahn, Dieter
Storbeck, Sonja
Layer, Gunhild
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-12-13
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Heme is an essential prosthetic group for many proteins involved in fundamental biological processes in all three domains of life.
In Eukaryota and Bacteria heme is formed via a conserved and well-studied biosynthetic pathway.
Surprisingly, in Archaea heme biosynthesis proceeds via an alternative route which is poorly understood.
In order to formulate a working hypothesis for this novel pathway, we searched 59 completely sequenced archaeal genomes for the presence of gene clusters consisting of established heme biosynthetic genes and colocalized conserved candidate genes.
Within the majority of archaeal genomes it was possible to identify such heme biosynthesis gene clusters.
From this analysis we have been able to identify several novel heme biosynthesis genes that are restricted to archaea.
Intriguingly, several of the encoded proteins display similarity to enzymes involved in heme d1 biosynthesis.
To initiate an experimental verification of our proposals two Methanosarcina barkeri proteins predicted to catalyze the initial steps of archaeal heme biosynthesis were recombinantly produced, purified, and their predicted enzymatic functions verified.
American Psychological Association (APA)
Storbeck, Sonja& Rolfes, Sarah& Raux-Deery, Evelyne& Warren, Martin J.& Jahn, Dieter& Layer, Gunhild. 2010. A Novel Pathway for the Biosynthesis of Heme in Archaea : Genome-Based Bioinformatic Predictions and Experimental Evidence. Archaea،Vol. 2010, no. 2010, pp.1-15.
https://search.emarefa.net/detail/BIM-451905
Modern Language Association (MLA)
Storbeck, Sonja…[et al.]. A Novel Pathway for the Biosynthesis of Heme in Archaea : Genome-Based Bioinformatic Predictions and Experimental Evidence. Archaea No. 2010 (2010), pp.1-15.
https://search.emarefa.net/detail/BIM-451905
American Medical Association (AMA)
Storbeck, Sonja& Rolfes, Sarah& Raux-Deery, Evelyne& Warren, Martin J.& Jahn, Dieter& Layer, Gunhild. A Novel Pathway for the Biosynthesis of Heme in Archaea : Genome-Based Bioinformatic Predictions and Experimental Evidence. Archaea. 2010. Vol. 2010, no. 2010, pp.1-15.
https://search.emarefa.net/detail/BIM-451905
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-451905