p53 Is a Key Regulator for Osthole-Triggered Cancer Pathogenesis
Joint Authors
Chen, Dar-Ren
Tsai, Cheng-Fang
Wang, Min-Ying
Huang, Ssu-Ming
Yeh, Wei-Lan
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-06-12
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Osthole has been reported to have antitumor activities via the induction of apoptosis and inhibition of cancer cell growth and metastasis.
However, the detailed molecular mechanisms underlying the anticancer effects of osthole in human colon cancer remain unclear.
In the present study, we have assessed osthole-induced cell death in two different human colon cancer cell lines, HCT116 and SW480.
Our results also showed that osthole activated proapoptotic signaling pathways in human colon cancer cells.
By using cell culture insert system, osthole reduced cell motility in both human colon cancer cell lines.
This study also provides evidence supporting the potential of osthole in p53 activation.
Expression of p53, an apoptotic protein, was remarkably upregulated in cells treated with osthole.
Importantly, the levels of phosphorylation of p53 on Ser15 (p-p53) and acetylation of p53 on Lys379 (acetyl-p53) were increased under osthole treatment.
Our results also demonstrated that p53 was activated followed by generation of reactive oxygen species (ROS) and activation of c-Jun N-terminal kinase (JNK).
Our study provides novel insights of p53-mediated responses under osthole treatment.
Taken together, we concluded that osthole induces cancer cell death and inhibits migratory activity in a controlled manner and is a promising candidate for antitumor drug development.
American Psychological Association (APA)
Huang, Ssu-Ming& Tsai, Cheng-Fang& Chen, Dar-Ren& Wang, Min-Ying& Yeh, Wei-Lan. 2014. p53 Is a Key Regulator for Osthole-Triggered Cancer Pathogenesis. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-451928
Modern Language Association (MLA)
Huang, Ssu-Ming…[et al.]. p53 Is a Key Regulator for Osthole-Triggered Cancer Pathogenesis. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-451928
American Medical Association (AMA)
Huang, Ssu-Ming& Tsai, Cheng-Fang& Chen, Dar-Ren& Wang, Min-Ying& Yeh, Wei-Lan. p53 Is a Key Regulator for Osthole-Triggered Cancer Pathogenesis. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-451928
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-451928