In Situ Degradation of Chitosan-Polygalacturonic AcidHydroxyapatite Nanocomposites in Cell Culture Media
Joint Authors
Khanna, Rohit
Katti, Dinesh R.
Katti, Kalpana S.
Source
International Journal of Polymer Science
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-06-03
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
The molecular and mechanical characteristics of in situ degradation behavior of chitosan-polygalacturonic acid/hydroxyapatite (Chi-PgA-HAP) nanocomposite films is investigated using Fourier Transform Infrared spectroscopy (FTIR), Atomic Force Microscopy (AFM), and modulus mapping techniques for up to 48 days of soaking in cell culture media.
The surface molecular structure of media-soaked samples changes over the course of 48 days of soaking, as indicated by significant changes in phosphate vibrations (1200–900 cm−1) indicating apatite formation.
Chitosan-Polygalacturonic acid polyelectrolyte complexes (PECs) govern structural integrity of Chi-PgA-HAP nanocomposites and FTIR spectra indicate that PECs remain intact until 48 days of soaking.
In situ AFM experiments on media-soaked samples indicate that soaking results in a change in topography and swelling proceeds differently at the initial soaking periods of about 8 days than for longer soaking.
In situ modulus mapping experiments are done on soaked samples by probing ∼1–3 nm of surface indicating elastic moduli of ∼4 GPa resulting from proteins adsorbed on Chi-PgA-HAP nanocomposites.
The elastic modulus decreases by ∼2 GPa over a long exposure to cell culture media (48 days).
Thus, as water enters the Chi-PgA-HAP sample, surface molecular interactions in Chi-PgA-HAP structure occur that result in swelling, causing small changes in nanoscale mechanical properties.
American Psychological Association (APA)
Khanna, Rohit& Katti, Kalpana S.& Katti, Dinesh R.. 2010. In Situ Degradation of Chitosan-Polygalacturonic AcidHydroxyapatite Nanocomposites in Cell Culture Media. International Journal of Polymer Science،Vol. 2010, no. 2010, pp.1-12.
https://search.emarefa.net/detail/BIM-451933
Modern Language Association (MLA)
Khanna, Rohit…[et al.]. In Situ Degradation of Chitosan-Polygalacturonic AcidHydroxyapatite Nanocomposites in Cell Culture Media. International Journal of Polymer Science No. 2010 (2010), pp.1-12.
https://search.emarefa.net/detail/BIM-451933
American Medical Association (AMA)
Khanna, Rohit& Katti, Kalpana S.& Katti, Dinesh R.. In Situ Degradation of Chitosan-Polygalacturonic AcidHydroxyapatite Nanocomposites in Cell Culture Media. International Journal of Polymer Science. 2010. Vol. 2010, no. 2010, pp.1-12.
https://search.emarefa.net/detail/BIM-451933
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-451933