Analysis of Genotype 1b Hepatitis C Virus IRES in Serum and Peripheral Blood Mononuclear Cells in Patients Treated with Interferon and Ribavirin
Joint Authors
Perlejewski, Karol
Fic, Maria
Demkow, Urszula
Pawełczyk, Agnieszka
Caraballo Cortés, Kamila
Horban, Andrzej
Berak, Hanna
Radkowski, Marek
Laskus, Tomasz
Bukowska-Ośko, Iwona
Płoski, Rafał
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-03
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Hepatitis C virus (HCV) highly conserved IRES (internal ribosome entry site) sequence, localized within the 5′-untranslated region (5′UTR), may determine viral properties like replication efficiency and cell tropism.
The aim of the present study was to characterize newly emerging 5′UTR variants in serum and peripheral blood mononuclear cells (PBMC) in chronic hepatitis C patients treated with interferon (IFN) and ribavirin and to identify their effect on IRES secondary structures.
The study group consisted of 87 patients infected with genotype 1b from whom serum and PBMC samples were collected at 9 time points (before, during, and after treatment).
New 5′UTR variants developed in 9 patients.
Out of the overall 14 new variants, 9 (64%) were found in PBMC.
HCV variants with decreased thermodynamic stability were identified only in PBMC and C183U mutation was the most common one in this compartment.
In conclusion, antiviral treatment may favor emergence of new 5′UTR variants both in blood and in PBMC compartments.
However, variants developing in the latter compartment were predicted to have lower thermodynamic stability of the IRES secondary structures compared to serum strains.
C-U change in position 183, which has not been described previously, might indicate viral adaptation to lymphoid cells.
American Psychological Association (APA)
Bukowska-Ośko, Iwona& Caraballo Cortés, Kamila& Pawełczyk, Agnieszka& Płoski, Rafał& Fic, Maria& Perlejewski, Karol…[et al.]. 2014. Analysis of Genotype 1b Hepatitis C Virus IRES in Serum and Peripheral Blood Mononuclear Cells in Patients Treated with Interferon and Ribavirin. BioMed Research International،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-451952
Modern Language Association (MLA)
Bukowska-Ośko, Iwona…[et al.]. Analysis of Genotype 1b Hepatitis C Virus IRES in Serum and Peripheral Blood Mononuclear Cells in Patients Treated with Interferon and Ribavirin. BioMed Research International No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-451952
American Medical Association (AMA)
Bukowska-Ośko, Iwona& Caraballo Cortés, Kamila& Pawełczyk, Agnieszka& Płoski, Rafał& Fic, Maria& Perlejewski, Karol…[et al.]. Analysis of Genotype 1b Hepatitis C Virus IRES in Serum and Peripheral Blood Mononuclear Cells in Patients Treated with Interferon and Ribavirin. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-451952
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-451952