Lipid Droplet Binding of Hepatitis C Virus Core Protein Genotype 3

Abstract EN

Background.

Hepatitis C virus (HCV) genotype 3 is known to cause steatosis (fatty liver) that is more frequent and severe than other genotypes.

We previously identified sequence elements within genotype 3 HCV Core domain 3 that were sufficient for lipid accumulation.

Aims.

We examined various genotype 3 Core domains for lipid droplet localization and compared the lipid droplet binding regions of domain 2 with a genotype 1 isolate.

Methods.

We generated HCV Core domain constructs fused with green fluorescent protein and performed immunofluorescence to visualize lipid droplets.

Results.

Constructs containing HCV Core domain 2 are appropriately localized to lipid droplets with varying degrees of efficiency.

When compared to genotype 1, there are polymorphisms within domain 2 that do not appear to alter lipid droplet localization.

Conclusions.

In summary, the differences in a steatosis-associated HCV Core genotype 3 isolate do not appear to involve altered lipid droplet localization.