Systemic Administration of CpG Oligodeoxynucleotide and Levamisole as Adjuvants for Gene-Gun-Delivered Antitumor DNA Vaccines
Joint Authors
Vajdová, Eva
Poláková, Ingrid
Sobotková, Eva
Šmahel, Michal
Source
Clinical and Developmental Immunology
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-10-18
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
DNA vaccines showed great promise in preclinical models of infectious and malignant diseases, but their potency was insufficient in clinical trials and is needed to be improved.
In this study, we tested systemic administration of two conventional adjuvants, synthetic oligodeoxynucleotide carrying immunostimulatory CpG motifs (CpG-ODN) and levamisole (LMS), and evaluated their effect on immune reactions induced by DNA vaccines delivered by a gene gun.
DNA vaccination was directed either against the E7 oncoprotein of human papillomavirus type 16 or against the BCR-ABL1 oncoprotein characteristic for chronic myeloid leukemia.
High doses of both adjuvants reduced activation of mouse splenic CD8+ T lymphocytes, but the overall antitumor effect was enhanced in both tumor models.
High-dose CpG-ODN exhibited a superior adjuvant effect in comparison with any combination of CpG-ODN with LMS.
In summary, our results demonstrate the benefit of combined therapy with gene-gun-delivered antitumor DNA vaccines and systemic administration of CpG-ODN or LMS.
American Psychological Association (APA)
Šmahel, Michal& Poláková, Ingrid& Sobotková, Eva& Vajdová, Eva. 2011. Systemic Administration of CpG Oligodeoxynucleotide and Levamisole as Adjuvants for Gene-Gun-Delivered Antitumor DNA Vaccines. Clinical and Developmental Immunology،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-452074
Modern Language Association (MLA)
Šmahel, Michal…[et al.]. Systemic Administration of CpG Oligodeoxynucleotide and Levamisole as Adjuvants for Gene-Gun-Delivered Antitumor DNA Vaccines. Clinical and Developmental Immunology No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-452074
American Medical Association (AMA)
Šmahel, Michal& Poláková, Ingrid& Sobotková, Eva& Vajdová, Eva. Systemic Administration of CpG Oligodeoxynucleotide and Levamisole as Adjuvants for Gene-Gun-Delivered Antitumor DNA Vaccines. Clinical and Developmental Immunology. 2011. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-452074
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-452074