Characterization of Ten Heterotetrameric NDP-Dependent Acyl-CoA Synthetases of the Hyperthermophilic Archaeon Pyrococcus furiosus

Joint Authors

Poole, Farris L.
Adams, Michael W. W.
Scott, Joseph W.

Source

Archaea

Issue

Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2014-02-11

Country of Publication

Egypt

No. of Pages

9

Main Subjects

Biology

Abstract EN

The hyperthermophilic archaeon Pyrococcus furiosus grows by fermenting peptides and carbohydrates to organic acids.

In the terminal step, acyl-CoA synthetase (ACS) isoenzymes convert acyl-CoA derivatives to the corresponding acid and conserve energy in the form of ATP.

ACS1 and ACS2 were previously purified from P.

furiosus and have α2β2 structures but the genome contains genes encoding three additional α-subunits.

The ten possible combinations of α and β genes were expressed in E.

coli and each resulted in stable and active α2β2 isoenzymes.

The α-subunit of each isoenzyme determined CoA-based substrate specificity and between them they accounted for the CoA derivatives of fourteen amino acids.

The β-subunit determined preference for adenine or guanine nucleotides.

The GTP-generating isoenzymes are proposed to play a role in gluconeogenesis by producing GTP for GTP-dependent phosphoenolpyruvate carboxykinase and for other GTP-dependent processes.

Transcriptional and proteomic data showed that all ten isoenzymes are constitutively expressed indicating that both ATP and GTP are generated from the metabolism of most of the amino acids.

A phylogenetic analysis showed that the ACSs of P.

furiosus and other members of the Thermococcales are evolutionarily distinct from those found throughout the rest of biology, including those of other hyperthermophilic archaea.

American Psychological Association (APA)

Scott, Joseph W.& Poole, Farris L.& Adams, Michael W. W.. 2014. Characterization of Ten Heterotetrameric NDP-Dependent Acyl-CoA Synthetases of the Hyperthermophilic Archaeon Pyrococcus furiosus. Archaea،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-452088

Modern Language Association (MLA)

Scott, Joseph W.…[et al.]. Characterization of Ten Heterotetrameric NDP-Dependent Acyl-CoA Synthetases of the Hyperthermophilic Archaeon Pyrococcus furiosus. Archaea No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-452088

American Medical Association (AMA)

Scott, Joseph W.& Poole, Farris L.& Adams, Michael W. W.. Characterization of Ten Heterotetrameric NDP-Dependent Acyl-CoA Synthetases of the Hyperthermophilic Archaeon Pyrococcus furiosus. Archaea. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-452088

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-452088