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Use of H19 Gene Regulatory Sequences in DNA-Based Therapy for Pancreatic Cancer
Joint Authors
Mizrahi, A.
Fellig, Y.
Buscail, L.
Scaiewicz, V.
Hochberg, Avraham
Abu-lail, R.
Birman, Tatiana
Galula, J.
Shneider, T.
Sorin, Vladimir
Czerniak, Abraham
Ohana, Patricia
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-10-28
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Pancreatic cancer is the eighth most common cause of death from cancer in the world, for which palliative treatments are not effective and frequently accompanied by severe side effects.
We propose a DNA-based therapy for pancreatic cancer using a nonviral vector, expressing the diphtheria toxin A chain under the control of the H19 gene regulatory sequences.
The H19 gene is an oncofetal RNA expressed during embryo development and in several types of cancer.
We tested the expression of H19 gene in patients, and found that 65% of human pancreatic tumors analyzed showed moderated to strong expression of the gene.
In vitro experiments showed that the vector was effective in reducing Luciferase protein activity on pancreatic carcinoma cell lines.
In vivo experiment results revealed tumor growth arrest in different animal models for pancreatic cancer.
Differences in tumor size between control and treated groups reached a 75% in the heterotopic model (P=.037) and 50% in the orthotopic model (P=.007).
In addition, no visible metastases were found in the treated group of the orthotopic model.
These results indicate that the treatment with the vector DTA-H19 might be a viable new therapeutic option for patients with unresectable pancreatic cancer.
American Psychological Association (APA)
Scaiewicz, V.& Sorin, Vladimir& Fellig, Y.& Birman, Tatiana& Mizrahi, A.& Galula, J.…[et al.]. 2010. Use of H19 Gene Regulatory Sequences in DNA-Based Therapy for Pancreatic Cancer. Journal of Oncology،Vol. 2010, no. 2010, pp.1-10.
https://search.emarefa.net/detail/BIM-452118
Modern Language Association (MLA)
Scaiewicz, V.…[et al.]. Use of H19 Gene Regulatory Sequences in DNA-Based Therapy for Pancreatic Cancer. Journal of Oncology No. 2010 (2010), pp.1-10.
https://search.emarefa.net/detail/BIM-452118
American Medical Association (AMA)
Scaiewicz, V.& Sorin, Vladimir& Fellig, Y.& Birman, Tatiana& Mizrahi, A.& Galula, J.…[et al.]. Use of H19 Gene Regulatory Sequences in DNA-Based Therapy for Pancreatic Cancer. Journal of Oncology. 2010. Vol. 2010, no. 2010, pp.1-10.
https://search.emarefa.net/detail/BIM-452118
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-452118