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Isolation and Identification of Sodium Fluoroacetate Degrading Bacteria from Caprine Rumen in Brazil
Joint Authors
Tadra-Sfeir, Michelle Z.
Junior, Felício G.
Camboim, Expedito Kennedy Alves
Riet-Correa, Franklin
de Andrade, Paulo Paes
de Melo, Marcia Almeida
McSweeney, Chris S.
Almeida, Arthur P.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-31
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Information Technology and Computer Science
Abstract EN
The objective of this paper was to report the isolation of two fluoroacetate degrading bacteria from the rumen of goats.
The animals were adult goats, males, crossbred, with rumen fistula, fed with hay, and native pasture.
The rumen fluid was obtained through the rumen fistula and immediately was inoculated 100 μL in mineral medium added with 20 mmol L−1 sodium fluoroacetate (SF), incubated at 39°C in an orbital shaker.
Pseudomonas fluorescens (strain DSM 8341) was used as positive control for fluoroacetate dehalogenase activity.
Two isolates were identified by 16S rRNA gene sequencing as Pigmentiphaga kullae (ECPB08) and Ancylobacter dichloromethanicus (ECPB09).
These bacteria degraded sodium fluoroacetate, releasing 20 mmol L−1 of fluoride ion after 32 hours of incubation in Brunner medium containing 20 mmol L−1 of SF.
There are no previous reports of fluoroacetate dehalogenase activity for P.
kullae and A.
dichloromethanicus.
Control measures to prevent plant intoxication, including use of fences, herbicides, or other methods of eliminating poisonous plants, have been unsuccessful to avoid poisoning by fluoroacetate containing plants in Brazil.
In this way, P.
kullae and A.
dichloromethanicus may be used to colonize the rumen of susceptible animals to avoid intoxication by fluoroacetate containing plants.
American Psychological Association (APA)
Camboim, Expedito Kennedy Alves& Almeida, Arthur P.& Tadra-Sfeir, Michelle Z.& Junior, Felício G.& de Andrade, Paulo Paes& McSweeney, Chris S.…[et al.]. 2012. Isolation and Identification of Sodium Fluoroacetate Degrading Bacteria from Caprine Rumen in Brazil. The Scientific World Journal،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-452123
Modern Language Association (MLA)
Camboim, Expedito Kennedy Alves…[et al.]. Isolation and Identification of Sodium Fluoroacetate Degrading Bacteria from Caprine Rumen in Brazil. The Scientific World Journal No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-452123
American Medical Association (AMA)
Camboim, Expedito Kennedy Alves& Almeida, Arthur P.& Tadra-Sfeir, Michelle Z.& Junior, Felício G.& de Andrade, Paulo Paes& McSweeney, Chris S.…[et al.]. Isolation and Identification of Sodium Fluoroacetate Degrading Bacteria from Caprine Rumen in Brazil. The Scientific World Journal. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-452123
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-452123