Decolorization of Solophenyl Red 3BL Polyazo Dye by Laccase-Mediator System : Optimization through Response Surface Methodology
Joint Authors
Ellouze-Ghorbel, Raoudha
Chaabouni-Ellouze, Semia
Neifar, Mohamed
Jaouani, Atef
Kamoun, Amel
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-08-02
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The decolorization of direct Solophenyl red 3BL (SR), a polyazo dye extensively used in textile industry was studied.
The Fomes fomentarius laccase alone did not decolorize SR.
The natural redox mediator, acetosyringone (AS), was necessary for decolorization to occur.
Box-Behnken design was used to evaluate the effects of three parameters, namely, enzyme concentration (0.5–2.5 U mL-1), redox mediator concentration (3–30 μM), and incubation time (1–24 h), on the SR decolorization yield.
The fitted mathematical model allowed us to plot response surfaces as well as isoresponse curves and to determine optimal decolorization conditions.
The results clearly indicated that the AS concentration was the main factor influencing the SR decolorization yield.
The selected optimal conditions were enzyme concentration 0.8 U mL-1, mediator concentration 33 μM, and time 14 h 30 min.
These conditions allowed 79.66% of SR decolorization versus 80.70% for the predicted value.
These results showed a promising future of applying laccase-AS system for industrial wastewater bioremediation.
American Psychological Association (APA)
Neifar, Mohamed& Jaouani, Atef& Kamoun, Amel& Ellouze-Ghorbel, Raoudha& Chaabouni-Ellouze, Semia. 2011. Decolorization of Solophenyl Red 3BL Polyazo Dye by Laccase-Mediator System : Optimization through Response Surface Methodology. Enzyme Research،Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-452194
Modern Language Association (MLA)
Neifar, Mohamed…[et al.]. Decolorization of Solophenyl Red 3BL Polyazo Dye by Laccase-Mediator System : Optimization through Response Surface Methodology. Enzyme Research No. 2011 (2011), pp.1-8.
https://search.emarefa.net/detail/BIM-452194
American Medical Association (AMA)
Neifar, Mohamed& Jaouani, Atef& Kamoun, Amel& Ellouze-Ghorbel, Raoudha& Chaabouni-Ellouze, Semia. Decolorization of Solophenyl Red 3BL Polyazo Dye by Laccase-Mediator System : Optimization through Response Surface Methodology. Enzyme Research. 2011. Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-452194
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-452194