Ferromagnetic Levan Composite : An Affinity Matrix to Purify Lectin
Joint Authors
Angeli, Renata
Almeida, Fábio C. L.
Maciel, Jackeline C.
de Carvalho Junior, Luiz Bezerra
Araújo, Flávia F. B.
Correia, Maria Tereza dos Santos
Valente, Ana Paula
Paiva, Patrícia Maria Guedes
Cassandra Breitenbach Barroso Coelho, Luana
Calazans, Glícia M. T.
da Silva, Maria da Paz Carvalho
da Paz, Nathalia V. N.
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-06-14
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
A simple and inexpensive procedure used magnetite and levan to synthesize a composite recovered by a magnetic field.
Lectins from Canavalia ensiformis (Con A) and Cratylia mollis (Cramoll 1 and Cramoll 1,4) did bind specifically to composite.
The magnetic property of derivative favored washing out contaminating proteins and recovery of pure lectins with glucose elution.
Cramoll 1 was purified by this affinity binding procedure in two steps instead of a previous three-step protocol with ammonium sulfate fractionation, affinity chromatography on Sephadex G-75, and ion exchange chromatography through a CM-cellulose column.
American Psychological Association (APA)
Angeli, Renata& da Paz, Nathalia V. N.& Maciel, Jackeline C.& Araújo, Flávia F. B.& Paiva, Patrícia Maria Guedes& Calazans, Glícia M. T.…[et al.]. 2009. Ferromagnetic Levan Composite : An Affinity Matrix to Purify Lectin. Journal of Biomedicine and Biotechnology،Vol. 2009, no. 2009, pp.1-6.
https://search.emarefa.net/detail/BIM-452202
Modern Language Association (MLA)
Angeli, Renata…[et al.]. Ferromagnetic Levan Composite : An Affinity Matrix to Purify Lectin. Journal of Biomedicine and Biotechnology No. 2009 (2009), pp.1-6.
https://search.emarefa.net/detail/BIM-452202
American Medical Association (AMA)
Angeli, Renata& da Paz, Nathalia V. N.& Maciel, Jackeline C.& Araújo, Flávia F. B.& Paiva, Patrícia Maria Guedes& Calazans, Glícia M. T.…[et al.]. Ferromagnetic Levan Composite : An Affinity Matrix to Purify Lectin. Journal of Biomedicine and Biotechnology. 2009. Vol. 2009, no. 2009, pp.1-6.
https://search.emarefa.net/detail/BIM-452202
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-452202
