WIPI-1 Positive Autophagosome-Like Vesicles Entrap Pathogenic Staphylococcus aureus for Lysosomal Degradation
Joint Authors
Krut, Oleg
Krönke, Martin
Yu, Wenqi
Mauthe, Mario
Götz, Friedrich
Robenek, Horst
Proikas-Cezanne, Tassula
Source
International Journal of Cell Biology
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-09
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Invading pathogens provoke the autophagic machinery and, in a process termed xenophagy, the host cell survives because autophagy is employed as a safeguard for pathogens that escaped phagosomes.
However, some pathogens can manipulate the autophagic pathway and replicate within the niche of generated autophagosome-like vesicles.
By automated fluorescence-based high content analyses, we demonstrate that Staphylococcus aureus strains (USA300, HG001, SA113) stimulate autophagy and become entrapped in intracellular PtdIns(3)P-enriched vesicles that are decorated with human WIPI-1, an essential PtdIns(3)P effector of canonical autophagy and membrane protein of both phagophores and autophagosomes.
Further, agr-positive S.
aureus (USA300, HG001) strains were more efficiently entrapped in WIPI-1 positive autophagosome-like vesicles when compared to agr-negative cells (SA113).
By confocal and electron microscopy we provide evidence that single- and multiple-Staphylococci entrapped undergo cell division.
Moreover, the number of WIPI-1 positive autophagosome-like vesicles entrapping Staphylococci significantly increased upon (i) lysosomal inhibition by bafilomycin A1 and (ii) blocking PIKfyve-mediated PtdIns(3,5)P2 generation by YM201636.
In summary, our results provide evidence that the PtdIns(3)P effector function of WIPI-1 is utilized during xenophagy of Staphylococcus aureus.
We suggest that invading S.
aureus cells become entrapped in autophagosome-like WIPI-1 positive vesicles targeted for lysosomal degradation in nonprofessional host cells.
American Psychological Association (APA)
Mauthe, Mario& Yu, Wenqi& Krut, Oleg& Krönke, Martin& Götz, Friedrich& Robenek, Horst…[et al.]. 2012. WIPI-1 Positive Autophagosome-Like Vesicles Entrap Pathogenic Staphylococcus aureus for Lysosomal Degradation. International Journal of Cell Biology،Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-452209
Modern Language Association (MLA)
Mauthe, Mario…[et al.]. WIPI-1 Positive Autophagosome-Like Vesicles Entrap Pathogenic Staphylococcus aureus for Lysosomal Degradation. International Journal of Cell Biology No. 2012 (2012), pp.1-13.
https://search.emarefa.net/detail/BIM-452209
American Medical Association (AMA)
Mauthe, Mario& Yu, Wenqi& Krut, Oleg& Krönke, Martin& Götz, Friedrich& Robenek, Horst…[et al.]. WIPI-1 Positive Autophagosome-Like Vesicles Entrap Pathogenic Staphylococcus aureus for Lysosomal Degradation. International Journal of Cell Biology. 2012. Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-452209
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-452209