Rosiglitazone Induces Mitochondrial Biogenesis in Differentiated Murine 3T3-L1 and C3H10T12 Adipocytes
Joint Authors
Qiu, Yang
Crosby, Renae M.
Strum, Jay C.
Ryan, Terence E.
Neill, Sue D.
Kashatus, Jennifer A.
Klein, Jean-Louis D.
Zhang, Vivian
Witherspoon, Sam M.
Bing, Nan
Moore, John T.
Xie, Mi
Jackson, Tymissha K.
Johnson, Jennifer H.
Waters, K. Michelle
Rong, James X.
Remlinger, Katja S.
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-10-15
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Growing evidence indicates that PPARγ agonists, including rosiglitazone (RSG), induce adipose mitochondrial biogenesis.
By systematically analyzing mitochondrial gene expression in two common murine adipocyte models, the current study aimed to further establish the direct role of RSG and capture temporal changes in gene transcription.
Microarray profiling revealed that in fully differentiated 3T3-L1 and C3H/10T1/2 adipocytes treated with RSG or DMSO vehicle for 1, 2, 4, 7, 24, and 48 hrs, RSG overwhelmingly increased mitochondrial gene transcripts time dependently.
The timing of the increases was consistent with the cascade of organelle biogenesis, that is, initiated by induction of transcription factor(s), followed by increases in the biosynthesis machinery, and then by increases in functional components.
The transcriptional increases were further validated by increased mitochondrial staining, citrate synthase activity, and O2 consumption, and were found to be associated with increased adiponectin secretion.
The work provided further insight on the mechanism of PPARγ-induced mitochondrial biogenesis in differentiated adipocytes.
American Psychological Association (APA)
Rong, James X.& Klein, Jean-Louis D.& Qiu, Yang& Xie, Mi& Johnson, Jennifer H.& Waters, K. Michelle…[et al.]. 2011. Rosiglitazone Induces Mitochondrial Biogenesis in Differentiated Murine 3T3-L1 and C3H10T12 Adipocytes. PPAR Research،Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-452233
Modern Language Association (MLA)
Rong, James X.…[et al.]. Rosiglitazone Induces Mitochondrial Biogenesis in Differentiated Murine 3T3-L1 and C3H10T12 Adipocytes. PPAR Research No. 2011 (2011), pp.1-11.
https://search.emarefa.net/detail/BIM-452233
American Medical Association (AMA)
Rong, James X.& Klein, Jean-Louis D.& Qiu, Yang& Xie, Mi& Johnson, Jennifer H.& Waters, K. Michelle…[et al.]. Rosiglitazone Induces Mitochondrial Biogenesis in Differentiated Murine 3T3-L1 and C3H10T12 Adipocytes. PPAR Research. 2011. Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-452233
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-452233