Exploring the Membrane Mechanism of the Bioactive Peptaibol Ampullosporin A Using Lipid Monolayers and Supported Biomimetic Membranes
Joint Authors
Rippa, Sonia
Desbat, Bernard
Béven, Laure
Chopineau, Joël
Rossi, Claire
Eid, Marguerita
Castano, Sabine
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-02-17
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Ampullosporin A is an antimicrobial, neuroleptic peptaibol, the behavior of which was investigated in different membrane mimetic environments made of egg yolk L-α-phosphatidylcholine.
In monolayers, the peptaibol adopted a mixed α/310-helical structure with an in-plane orientation.
The binding step was followed by the peptide insertion into the lipid monolayer core.
The relevance of the inner lipid leaflet nature was studied by comparing ampullosporin binding on a hybrid bilayer, in which this leaflet was a rigid alkane layer, and on supported fluid lipid bilayers.
The membrane binding was examined by surface plasmon resonance spectroscopy and the effect on lipid dynamics was explored using fluorescence recovery after photobleaching.
In the absence of voltage and at low concentration, ampullosporin A substantially adsorbed onto lipid surfaces and its interaction with biomimetic models was strongly modified depending on the inner leaflet structure.
At high concentration, ampullosporin A addition led to the lipid bilayers disruption.
American Psychological Association (APA)
Eid, Marguerita& Rippa, Sonia& Castano, Sabine& Desbat, Bernard& Chopineau, Joël& Rossi, Claire…[et al.]. 2011. Exploring the Membrane Mechanism of the Bioactive Peptaibol Ampullosporin A Using Lipid Monolayers and Supported Biomimetic Membranes. Journal of Biophysics،Vol. 2010, no. 2010, pp.1-12.
https://search.emarefa.net/detail/BIM-452255
Modern Language Association (MLA)
Eid, Marguerita…[et al.]. Exploring the Membrane Mechanism of the Bioactive Peptaibol Ampullosporin A Using Lipid Monolayers and Supported Biomimetic Membranes. Journal of Biophysics No. 2010 (2010), pp.1-12.
https://search.emarefa.net/detail/BIM-452255
American Medical Association (AMA)
Eid, Marguerita& Rippa, Sonia& Castano, Sabine& Desbat, Bernard& Chopineau, Joël& Rossi, Claire…[et al.]. Exploring the Membrane Mechanism of the Bioactive Peptaibol Ampullosporin A Using Lipid Monolayers and Supported Biomimetic Membranes. Journal of Biophysics. 2011. Vol. 2010, no. 2010, pp.1-12.
https://search.emarefa.net/detail/BIM-452255
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-452255