Yeast Extract Promotes Cell Growth and Induces Production of Polyvinyl Alcohol-Degrading Enzymes

Abstract EN

Polyvinyl alcohol-degrading enzymes (PVAases) have a great potential in bio-desizing processes for its low environmental impact and low energy consumption.

In this study, the effect of yeast extract on PVAases production was investigated.

A strategy of four-point yeast extract addition was developed and applied to maximize cell growth and PVAases production.

As a result, the maximum dry cell weight achieved was 1.48 g/L and the corresponding PVAases activity was 2.99 U/mL, which are 46.5% and 176.8% higher than the control, respectively.

Applying this strategy in a 7 L fermentor increased PVAases activity to 3.41 U/mL.

Three amino acids (glycine, serine, and tyrosine) in yeast extract play a central role in the production of PVAases.

These results suggest that the new strategy of four-point yeast extract addition could benefit PVAases production.