Immobilization and Characterization of a Recombinant Thermostable Lipase (Pf2001) from Pyrococcus furiosus on Supports with Different Degrees of Hydrophobicity
Joint Authors
Branco, Roberta Vieira
Freire, Denise Maria Guimarães
Estrada Gutarra, Melissa Limoeiro
Almeida, Rodrigo Volcan
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-10-28
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
We studied the immobilization of a recombinant thermostable lipase (Pf2001Δ60) from the hyperthermophilic archaeon Pyrococcus furiosus on supports with different degrees of hydrophobicity: butyl Sepabeads and octadecyl Sepabeads.
The enzyme was strongly adsorbed in both supports.
When it was adsorbed on these supports, the enzyme showed 140 and 237% hyperactivation, respectively.
The assessment of storage stability showed that the octadecyl Sepabeads immobilized enzyme showed 100% of residual activity after 30 days of storage.
However, the greatest stability at 70∘C was obtained in butyl Sepabeads immobilized enzyme, which retained 77% activity after 1 hour incubation.
The maximum activity of the immobilized preparations was obtained with the pH between 6 and 7, at 70∘C.
Thus, this study achieved a new extremophilic biocatalyst with greater stability, for use in several biotechnological processes.
American Psychological Association (APA)
Branco, Roberta Vieira& Estrada Gutarra, Melissa Limoeiro& Freire, Denise Maria Guimarães& Almeida, Rodrigo Volcan. 2010. Immobilization and Characterization of a Recombinant Thermostable Lipase (Pf2001) from Pyrococcus furiosus on Supports with Different Degrees of Hydrophobicity. Enzyme Research،Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-452318
Modern Language Association (MLA)
Branco, Roberta Vieira…[et al.]. Immobilization and Characterization of a Recombinant Thermostable Lipase (Pf2001) from Pyrococcus furiosus on Supports with Different Degrees of Hydrophobicity. Enzyme Research No. 2010 (2010), pp.1-8.
https://search.emarefa.net/detail/BIM-452318
American Medical Association (AMA)
Branco, Roberta Vieira& Estrada Gutarra, Melissa Limoeiro& Freire, Denise Maria Guimarães& Almeida, Rodrigo Volcan. Immobilization and Characterization of a Recombinant Thermostable Lipase (Pf2001) from Pyrococcus furiosus on Supports with Different Degrees of Hydrophobicity. Enzyme Research. 2010. Vol. 2010, no. 2010, pp.1-8.
https://search.emarefa.net/detail/BIM-452318
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-452318