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A C21-Steroidal Glycoside Isolated from the Roots of Cynanchum auriculatum Induces Cell Cycle Arrest and Apoptosis in Human Gastric Cancer SGC-7901 Cells
Joint Authors
Yang, Bo
Ye, Liang-Fei
Lu, Hong
Zhang, Ru-Song
Zhang, Shui-Juan
Wang, Yi-Qi
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-19
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Caudatin 3-O-β-D-cymaropyranosyl-(1→4)-β-D-oleandropyranosyl-(1→4)-β-D-cymaropyranosyl-(1→4)-β-D-cymaropyranoside (CGII) is one of the C21-steroidal glycosides isolated from the roots of Cynanchum auriculatum ROYLE ex WIGHT.
This study aimed to determine the cell growth, cell proliferation, and apoptotic cell death of human gastric cancer cells after CGII treatment.
MTT assay was used to determine cell growth; fluorescence-activated cell sorting analysis was used to evaluate cell cycle distribution and apoptotic cell death.
Immunoblotting was applied for measuring the expression of proteins involved in the cell cycle progression.
The activities of caspase-3, -8, and -9 were detected by colorimetric caspase activity assays.
CGII inhibited cell growth of human gastric cancer SGC-7901 cells in a concentration- and time-dependent manner.
Treatment of SGC-7901 cells with CGII resulted in G1 phase cell cycle arrest, accompanied with decreased expression of cyclin D1 and cyclin-dependent kinases 4 and 6.
CGII induced cell apoptosis and activated caspase-3, caspase-8, and caspase-9.
In contrast, pan-caspase inhibitor z-VAD-fmk partially abolished the CGII-induced growth inhibition of SGC-7901 cells.
In conclusion, CGII inhibits cell growth of human gastric cancer cells by inducing G1 phase cell cycle arrest and caspase-dependent apoptosis cascades.
American Psychological Association (APA)
Wang, Yi-Qi& Zhang, Shui-Juan& Lu, Hong& Yang, Bo& Ye, Liang-Fei& Zhang, Ru-Song. 2013. A C21-Steroidal Glycoside Isolated from the Roots of Cynanchum auriculatum Induces Cell Cycle Arrest and Apoptosis in Human Gastric Cancer SGC-7901 Cells. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-452374
Modern Language Association (MLA)
Wang, Yi-Qi…[et al.]. A C21-Steroidal Glycoside Isolated from the Roots of Cynanchum auriculatum Induces Cell Cycle Arrest and Apoptosis in Human Gastric Cancer SGC-7901 Cells. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-7.
https://search.emarefa.net/detail/BIM-452374
American Medical Association (AMA)
Wang, Yi-Qi& Zhang, Shui-Juan& Lu, Hong& Yang, Bo& Ye, Liang-Fei& Zhang, Ru-Song. A C21-Steroidal Glycoside Isolated from the Roots of Cynanchum auriculatum Induces Cell Cycle Arrest and Apoptosis in Human Gastric Cancer SGC-7901 Cells. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-7.
https://search.emarefa.net/detail/BIM-452374
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-452374