Original Approach for Automated Quantification of Antinuclear Autoantibodies by Indirect Immunofluorescence
Joint Authors
Bongrand, Pierre
Jourde-Chiche, Noémie
Bardin, Nathalie
Bertin, Daniel
Source
Clinical and Developmental Immunology
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-18
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Introduction.
Indirect immunofluorescence (IIF) is the gold standard method for the detection of antinuclear antibodies (ANA) which are essential markers for the diagnosis of systemic autoimmune rheumatic diseases.
For the discrimination of positive and negative samples, we propose here an original approach named Immunofluorescence for Computed Antinuclear antibody Rational Evaluation (ICARE) based on the calculation of a fluorescence index (FI).
Methods.
We made comparison between FI and visual evaluations on 237 consecutive samples and on a cohort of 25 patients with SLE.
Results.
We obtained very good technical performance of FI (95% sensitivity, 98% specificity, and a kappa of 0.92), even in a subgroup of weakly positive samples.
A significant correlation between quantification of FI and IIF ANA titers was found (Spearman's ρ=0.80, P<0.0001).
Clinical performance of ICARE was validated on a cohort of patients with SLE corroborating the fact that FI could represent an attractive alternative for the evaluation of antibody titer.
Conclusion.
Our results represent a major step for automated quantification of IIF ANA, opening attractive perspectives such as rapid sample screening and laboratory standardization.
American Psychological Association (APA)
Bertin, Daniel& Jourde-Chiche, Noémie& Bongrand, Pierre& Bardin, Nathalie. 2013. Original Approach for Automated Quantification of Antinuclear Autoantibodies by Indirect Immunofluorescence. Clinical and Developmental Immunology،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-452487
Modern Language Association (MLA)
Bertin, Daniel…[et al.]. Original Approach for Automated Quantification of Antinuclear Autoantibodies by Indirect Immunofluorescence. Clinical and Developmental Immunology No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-452487
American Medical Association (AMA)
Bertin, Daniel& Jourde-Chiche, Noémie& Bongrand, Pierre& Bardin, Nathalie. Original Approach for Automated Quantification of Antinuclear Autoantibodies by Indirect Immunofluorescence. Clinical and Developmental Immunology. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-452487
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-452487