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Effect of Inflammatory Mediators on ATP Release of Human Urothelial RT4 Cells
Joint Authors
Mansfield, Kylie J.
Hughes, Jessica R.
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-15
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Inflammation is an important contributor to the aetiology of a number of bladder dysfunctions including interstitial cystitis, painful bladder syndrome, and overactive bladder.
The aim of this study was to examine the effects of inflammatory mediators on urothelial ATP release.
Human urothelial RT4 cells were exposed to normal buffer or varying concentrations of inflammatory mediators (bradykinin, histamine, and serotonin) in the presence or absence of hypotonic stretch stimuli (1 : 2 dilution of Krebs-Henseleit buffer).
Others have demonstrated that bradykinin increased stretch-induced ATP release; however, we observed no change in control or stretch-induced ATP release with bradykinin.
Pretreatment of RT4 cells with histamine or serotonin decreased stretch-induced ATP release (P=0.037, P=0.040, resp.).
Previous studies have demonstrated increased ATP release in response to inflammation utilising whole bladder preparations in contrast to our simple model of cultured urothelial cells.
The current study suggests that it is unlikely that there is a direct interaction between the release of inflammatory mediators and increased ATP release, but rather more complex interactions occurring in response to inflammation that lead to increased bladder sensation.
American Psychological Association (APA)
Mansfield, Kylie J.& Hughes, Jessica R.. 2014. Effect of Inflammatory Mediators on ATP Release of Human Urothelial RT4 Cells. BioMed Research International،Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-452541
Modern Language Association (MLA)
Mansfield, Kylie J.& Hughes, Jessica R.. Effect of Inflammatory Mediators on ATP Release of Human Urothelial RT4 Cells. BioMed Research International No. 2014 (2014), pp.1-6.
https://search.emarefa.net/detail/BIM-452541
American Medical Association (AMA)
Mansfield, Kylie J.& Hughes, Jessica R.. Effect of Inflammatory Mediators on ATP Release of Human Urothelial RT4 Cells. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-6.
https://search.emarefa.net/detail/BIM-452541
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-452541