Regulation of Chemokine Production via Oxidative Pathway in HeLa Cells

Joint Authors

Matayoshi, Akira
Nakasone, Toshiyuki
Makishi, Shoko
Liang, Feixin
Sunagawa, Nao
Sunakawa, Hajime
Takemoto, Hiroyuki
Phonaphonh, Thongsavanh
Kina, Shinichiro

Source

Mediators of Inflammation

Issue

Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-5, 5 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2010-01-27

Country of Publication

Egypt

No. of Pages

5

Main Subjects

Diseases

Abstract EN

Inflammation is associated with disease progression and, by largely unknown mechanisms, has been said to drive oncogenesis.

At inflamed sites, neutrophils deploy a potent antimicrobial arsenal that includes proteinases, antimicrobial peptides, and ROS.

Reactive oxygen species (ROSs) induce chemokines.

In the present study, the concentrations of IL-8 in culture supernatants of HeLa cells treated with ROS were determined by enzyme-linked immunosorbent assay.

We used o-phenanthroline to deplete Fe2+ in order to investigate the mechanisms through which ROSs induce IL-8 secretion in our system.

The iron chelator o-phenanthroline effectively inhibited H2O2-induced ERK2 activation.

Enzyme-linked immunosorbent assays showed that IL-8 protein secretion was elevated in ROS-treated HeLa cells.

When Fe2+ was removed from these cells, IL-8 secretion was inhibited.

Collectively, these results indicate that Fe2+-mediated Erk pathway activation is an important signal transduction pathway in ROS-induced IL-8 secretion in epithelial cells.

American Psychological Association (APA)

Kina, Shinichiro& Nakasone, Toshiyuki& Takemoto, Hiroyuki& Matayoshi, Akira& Makishi, Shoko& Sunagawa, Nao…[et al.]. 2010. Regulation of Chemokine Production via Oxidative Pathway in HeLa Cells. Mediators of Inflammation،Vol. 2009, no. 2009, pp.1-5.
https://search.emarefa.net/detail/BIM-452629

Modern Language Association (MLA)

Kina, Shinichiro…[et al.]. Regulation of Chemokine Production via Oxidative Pathway in HeLa Cells. Mediators of Inflammation No. 2009 (2009), pp.1-5.
https://search.emarefa.net/detail/BIM-452629

American Medical Association (AMA)

Kina, Shinichiro& Nakasone, Toshiyuki& Takemoto, Hiroyuki& Matayoshi, Akira& Makishi, Shoko& Sunagawa, Nao…[et al.]. Regulation of Chemokine Production via Oxidative Pathway in HeLa Cells. Mediators of Inflammation. 2010. Vol. 2009, no. 2009, pp.1-5.
https://search.emarefa.net/detail/BIM-452629

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-452629