Reversed-Phase Liquid Chromatographic Separation and Determination of Ni(II)‎, Cu(II)‎, Pd(II)‎, and Ag(I)‎ Using 2-Pyrrolecarboxaldehyde-4-phenylsemicarbazone as a Complexing Reagent

Abstract EN

This paper reports the utilization of 2-pyrrolecarboxaldehyde-4-phenylsemicarbazone (PPS) as a complexing reagent for the simultaneous determination and separation of Ni(II), Cu(II), Pd(II), and Ag(I) by reversed-phase high-performance liquid chromatography with UV detector.

A good separation was achieved using Microsorb C18 column (150 × 4.6 mm i.d.) with a mobile phase consisted of methanol : acetonitrile : water : sodium acetate (1 mM) (68 : 6.5 : 25 : 0.5 v/v/v/v) at a flow rate of 1 mL/min.

The detection was performed at 280 nm.

The linear calibration range was 2–10 μg/mL for all metal ions.

The detection limits (S/N = 3) were 80 pg/mL for Ni(II), 0.8 ng/mL for Cu(II), 0.16 ng/mL for Pd(II), and 0.8 ng/mL for Ag(I).

The applicability and the accuracy of the developed method were estimated by the analysis of Ni(II) in hydrogenated oil (ghee) samples and Pd(II) in palladium charcoal.