Qushi Huayu Decoction Inhibits Hepatic Lipid Accumulation by Activating AMP-Activated Protein Kinase In Vivo and In Vitro
Joint Authors
Hu, Yi-Yang
Xu, Lin
Wang, Wen-jing
Feng, Qin
Tang, Ya-jun
Meng, Sheng-xi
Peng, Jing-hua
Huang, Cheng
Zhang, Yu-quan
Gou, Xiao-jun
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-03-19
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Qushi Huayu Decoction (QHD), a Chinese herbal formula, has been proven effective on alleviating nonalcoholic fatty liver disease (NAFLD) in human and rats.
The present study was conducted to investigate whether QHD could inhibit hepatic lipid accumulation by activating AMP-activated protein kinase (AMPK) in vivo and in vitro.
Nonalcoholic fatty liver (NAFL) model was duplicated with high-fat diet in rats and with free fatty acid (FFA) in L02 cells.
In in vivo experimental condition, QHD significantly decreased the accumulation of fatty droplets in livers, lowered low-density lipoprotein cholesterol (LDL-c), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) levels in serum.
Moreover, QHD supplementation reversed the HFD-induced decrease in the phosphorylation levels of AMPK and acetyl-CoA carboxylase (ACC) and decreased hepatic nuclear protein expression of sterol regulatory element-binding protein-1 (SREBP-1) and carbohydrate-responsive element-binding protein (ChREBP) in the liver.
In in vitro, QHD-containing serum decreased the cellular TG content and alleviated the accumulation of fatty droplets in L02 cells.
QHD supplementation reversed the FFA-induced decrease in the phosphorylation levels of AMPK and ACC and decreased the hepatic nuclear protein expression of SREBP-1 and ChREBP.
Overall results suggest that QHD has significant effect on inhibiting hepatic lipid accumulation via AMPK pathway in vivo and in vitro.
American Psychological Association (APA)
Feng, Qin& Gou, Xiao-jun& Meng, Sheng-xi& Huang, Cheng& Zhang, Yu-quan& Tang, Ya-jun…[et al.]. 2013. Qushi Huayu Decoction Inhibits Hepatic Lipid Accumulation by Activating AMP-Activated Protein Kinase In Vivo and In Vitro. Evidence-Based Complementary and Alternative Medicine،Vol. 2013, no. 2013, pp.1-14.
https://search.emarefa.net/detail/BIM-452685
Modern Language Association (MLA)
Feng, Qin…[et al.]. Qushi Huayu Decoction Inhibits Hepatic Lipid Accumulation by Activating AMP-Activated Protein Kinase In Vivo and In Vitro. Evidence-Based Complementary and Alternative Medicine No. 2013 (2013), pp.1-14.
https://search.emarefa.net/detail/BIM-452685
American Medical Association (AMA)
Feng, Qin& Gou, Xiao-jun& Meng, Sheng-xi& Huang, Cheng& Zhang, Yu-quan& Tang, Ya-jun…[et al.]. Qushi Huayu Decoction Inhibits Hepatic Lipid Accumulation by Activating AMP-Activated Protein Kinase In Vivo and In Vitro. Evidence-Based Complementary and Alternative Medicine. 2013. Vol. 2013, no. 2013, pp.1-14.
https://search.emarefa.net/detail/BIM-452685
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-452685