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Data-Driven Compensation for Flow Cytometry of Solid Tissues
Joint Authors
Hoop, Sjack
Rodijnen, Nickolaas Maria van
Nap, Marius
Pieters, Math
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-09-06
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Propidium Iodide is a fluorochrome that is used to measure the DNA content of individual cells, taken from solid tissues, with a flow cytometer.
Compensation for spectral cross-over of this fluorochrome still leads to compensation results that are depending on operator experience.
We present a data-driven compensation (DDC) algorithm that is designed to automatically compensate combined DNA phenotype flow cytometry acquisitions.
The generated compensation values of the DDC algorithm are validated by comparison with manually determined compensation values.
The results show that (1) compensation of two-color flow cytometry leads to comparable results using either manual compensation or the DDC method; (2) DDC can calculate sample-specific compensation trace lines; (3) the effects of two different approaches to calculate compensation values can be visualized within one sample.
We conclude that the DDC algorithm contributes to the standardization of compensation for spectral cross-over in flow cytometry of solid tissues.
American Psychological Association (APA)
Rodijnen, Nickolaas Maria van& Pieters, Math& Hoop, Sjack& Nap, Marius. 2011. Data-Driven Compensation for Flow Cytometry of Solid Tissues. Advances in Bioinformatics،Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-452710
Modern Language Association (MLA)
Rodijnen, Nickolaas Maria van…[et al.]. Data-Driven Compensation for Flow Cytometry of Solid Tissues. Advances in Bioinformatics No. 2011 (2011), pp.1-11.
https://search.emarefa.net/detail/BIM-452710
American Medical Association (AMA)
Rodijnen, Nickolaas Maria van& Pieters, Math& Hoop, Sjack& Nap, Marius. Data-Driven Compensation for Flow Cytometry of Solid Tissues. Advances in Bioinformatics. 2011. Vol. 2011, no. 2011, pp.1-11.
https://search.emarefa.net/detail/BIM-452710
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-452710